DISTRIBUTION OF THE SGLT1 NA+/GLUCOSE COTRANSPORTER AND MESSENGER-RNA ALONG THE CRYPT-VILLUS AXIS OF RABBIT SMALL-INTESTINE
DISTRIBUTION OF THE SGLT1 NA+/GLUCOSE COTRANSPORTER AND MESSENGER-RNA ALONG THE CRYPT-VILLUS AXIS OF RABBIT SMALL-INTESTINE
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DOI：
10.1016/0006-291x(91)92067-t
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发表时间：
1991-12-31
影响因子：
3.1
通讯作者：
WRIGHT, EM
中科院分区：
文献类型：
--
作者：
HWANG, ES;HIRAYAMA, BA;WRIGHT, EM
使用原位杂交和免疫细胞化学技术检测了沿着兔小肠隐窝-绒毛轴的Na+葡萄糖协同转运蛋白（SGLT1）mRNA和蛋白的表达。我们在绒毛细胞中检测到 mRNA，但在隐窝中未检测到。从绒毛基部到顶端，mRNA 丰度增加了 6 倍。 SGLT1 蛋白仅限于成熟肠上皮细胞的刷状缘。我们认为，跨绒毛尖端的高糖转运率是由于成熟肠上皮细胞中 SGLT1 基因的转录、SGLT mRNA 的后续翻译以及功能性 SGLT1 转运蛋白直接插入绒毛尖端这些细胞的刷状缘膜中所致。
The expression of the Na+ glucose cotransporter (SGLT1) mRNA and protein along the crypt-villus axis of the rabbit small intestine was examined using in situ hybridization and immunocytochemical techniques. We detected mRNA in the cells on the villus, but not in the crypts. and the mRNA abundance increased 6-fold from the base to the tip of the villus. SGLT1 protein was restricted to the brush borders of mature enterocytes. We suggest that the high rate of sugar transport across the tips of the villus is due to the transcription of the SGLT1 gene in mature enterocytes, the subsequent translation of SGLT mRNA, and the insertion direct of the functional SGLT1 transporter into the brush border membrane of these cells lining the villus tip.