Intertrigeminal region attenuates reflex apnea and stabilizes respiratory pattern in rats
Intertrigeminal region attenuates reflex apnea and stabilizes respiratory pattern in rats
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DOI：
10.1016/s0006-8993(03)02587-3
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发表时间：
2003-06-13
期刊：
影响因子：
2.9
通讯作者：
Carley, DW
中科院分区：
文献类型：
--
作者：
Radulovacki, M;Pavlovic, S;Carley, DW
本研究的目的是确定新描述的涉及脑桥三叉神经间区域（ITR）的解剖通路（Chamberlin 和 Saper，1998）是否在介导或调节迷走神经诱导的反射性呼吸暂停中具有生理作用。我们探讨了 ITR 对 10 只麻醉大鼠静脉注射 5-HT 引起的迷走神经反射性呼吸暂停的影响。将导管插入动物的股静脉以给予5-HT（0.00375mg）并通过压电晶体记录呼吸。使用多管移液器将谷氨酸（5-10 nl，10 mM）、犬尿酸（10 nl，50 mM，谷氨酸受体拮抗剂）和红色染料单侧和双侧压力注射到ITR中。静脉注射 5-HT 会立即导致 3 秒呼吸暂停。将谷氨酸微量注射到 ITR 中会产生呼吸暂停，而微量注射犬尿酸则会阻断谷氨酸的作用。谷氨酸拮抗作用后，随后施用 5-HT 会产生更长持续时间的呼吸暂停（8 秒）。两只动物的急性脑桥延髓横断导致 5-HT 诱导的呼吸暂停的延长时间甚至更长。我们得出结论，ITR 在呼吸中的生理作用是减弱迷走神经诱导的反射性呼吸暂停。这一发现与脑桥结构在自主活动（包括呼吸、心率和血压调节）中的一般调节作用一致。此外，我们的数据表明，独立于脑桥结构的髓质回路足以产生 5-HT 诱导的反射性呼吸暂停。 (C) 2003 Elsevier Science B.V. 保留所有权利。
The goal of the present study was to determine whether the newly described anatomical pathway involving the pontine inter-trigeminal region (ITR), (Chamberlin and Saper, 1998), has a physiological role in mediating or modulating vagally-induced reflex apnea. We explored the ITR impact on vagal reflex apnea elicited by intravenuously injected 5-HT in ten anesthetized rats. The animals had a catheter inserted into the femoral vein for administration of 5-HT (0.00375 mg) and respiration was recorded by piezo-electric crystal. Multibarrel pipettes were used to pressure inject glutamate (5-10 nl, 10 mM), kynurenic acid (10 nl, 50 mM, a glutamate receptor antagonist), and red dye into the ITR, unilaterally and bilaterally. Intravenous administration of 5-HT produced an immediate 3-s apnea. Microinjections of glutamate into the ITR produced apneas, while microinjections of kynurenic acid blocked the glutamate effect. Following glutamate antagonism, subsequent administration of 5-HT produced apneas of much longer duration (8 s). Acute pontomedullary transection in two animals yielded even greater prolongation of 5-HT-induced apnea. We conclude that a physiological role for the ITR in respiration is to attenuate vagally-induced reflex apneas. This finding is in agreement with the general modulatory role of pontine structures in autonomic activities including respiration, heart rate and regulation of blood pressure. In addition, our data indicate that medullary circuits, independent of pontine structures, are sufficient to produce 5-HT induced reflex apnea. (C) 2003 Elsevier Science B.V. All rights reserved.