LAMBDA-REPLACEMENT VECTORS CARRYING POLYLINKER SEQUENCES
LAMBDA-REPLACEMENT VECTORS CARRYING POLYLINKER SEQUENCES
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DOI：
10.1016/s0022-2836(83)80190-9
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
MURRAY, N
中科院分区：
文献类型：
--
作者：
FRISCHAUF, AM;LEHRACH, H;MURRAY, N
为了简化基因组文库的构建和筛选，我们建立了一个新的.lambda。制备替代载体（EMBL1、EMBL2、EMBL3、EMBL4）和琥珀色突变衍生物（EMBL3 Sam、EMBL3 AamBam、EMBL3 AamSam）。这些载体具有较大的容量和中间片段两侧的多链接序列。多连接体允许选择克隆酶，特别是在克隆Sau3A部分酶的情况下，通过侧面SalI （EMBL3）或EcoRI （EMBL4）位点切除整个插入物。带插入体的噬菌体可以通过生物化学（特别是EMBL3）或基因上的Spi-表型进行选择。EMBL3载体的琥珀色衍生物允许应用基于同源重组事件产物选择的遗传筛选程序，以及选择性克隆与supF基因相关的DNA序列。
To simplify the construction and screening of genomic libraries, a new family of .lambda. replacement vectors (EMBL1, EMBL2, EMBL3, EMBL4) and derivatives containing amber mutations (EMBL3 Sam, EMBL3 AamBam, EMBL3 AamSam were made. These vectors have a large capacity and polylinker sequences flanking the middle fragment. The polylinkers allow a choice of cloning enzymes and, especially useful in the case of cloning of Sau3A partial digests, the excision of the entire insert by flanking SalI (EMBL3) or EcoRI (EMBL4) sites. Phages with inserts can be selected either biochemically (particularly EMBL3) or genetically by their Spi- phenotype. Amber derivatives of the EMBL3 vector allow the application of genetic screening procedures based on selection for the products of homologous recombination events, and for the selective cloning of DNA sequences linked to supF genes.