Lysophosphatidic acid-induced interleukin-1β expression is mediated through Gi/Rho and the generation of reactive oxygen species in macrophages
Lysophosphatidic acid-induced interleukin-1β expression is mediated through Gi/Rho and the generation of reactive oxygen species in macrophages
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DOI：
10.1007/s11373-007-9223-x
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发表时间：
2008-05-01
影响因子：
11
通讯作者：
Lee, Hsinyu
中科院分区：
文献类型：
--
作者：
Chang, Chi-Lun;Lin, Mu-En;Lee, Hsinyu
溶血磷脂酸 (LPA) 是一种富含血小板和轻度氧化的低密度脂蛋白的低分子量溶血磷脂，已知可通过与其同源受体结合来调节炎症和动脉粥样硬化。在本研究中，我们报道 LPA 上调小鼠 J774A.1 巨噬细胞中白细胞介素 1 β (IL-1 β) 的表达。通过使用药理学抑制剂，表明 G(i)/Rho 激活和随后的活性氧 (ROS) 产生参与 IL-1 β 诱导。此外，还在人原代巨噬细胞中观察到 LPA 诱导 IL-1 β。总之，LPA 通过影响巨噬细胞行为参与炎症过程。
Lysophophatidic acid (LPA), a low-molecular-weight lysophospholipid enriched in platelets and mildly oxidized low-density lipoproteins, is known to regulate inflammation and atherosclerosis by binding to its cognate receptors. In this study, we reported that LPA upregulated interleukin-1 beta (IL-1 beta) expression in mouse J774A.1 macrophages. By using pharmacological inhibitors, it was suggested that G(i)/Rho activation and subsequent reactive oxygen species (ROS) production were involved in IL-1 beta induction. In addition, IL-1 beta induction by LPA was also observed in human primary macrophages. In summary, LPA is involved in the processes of inflammation by affecting macrophage behavior.