Growth of kidney epithelial cells in culture: evidence for autocrine control.
Growth of kidney epithelial cells in culture: evidence for autocrine control.
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培养物中肾上皮细胞的生长：自分泌控制的证据。
DOI：
10.1152/ajpcell.1984.246.3.c351
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Toback,FG
中科院分区：
文献类型：
--
作者：
Mordan,LJ;Toback,FG
在K+缺乏的动物中刺激肾脏生长的因素尚不清楚。用肾上皮细胞(BSC-1系)的培养来研究这一现象，因为它们在含有降低K+浓度的培养液中生长加速。我们测试了低K+介质诱导的生长是由细胞产生的因子介导的假设，即受自分泌控制。将低K+(3.2 mM)或对照(5.4 mM)条件培养液置于BSC-1细胞融合培养1h后收集。低K+条件培养液的K+浓度通过添加KCl值调整到控制值。该条件培养液对新鲜培养的细胞生长的刺激程度与非条件低K+培养液相同。当K+浓度为3.2 mM时，培养基促生长活性最强。K+浓度为5.4 mM的低K+条件培养液，需要6h才能保证细胞促进增殖。低K+条件培养液中的促生长活性可被细胞产生的一种纯化的生长抑制物拮抗。这些观察结果与自分泌产物对生长有相反作用的假设是一致的，可以调节肾上皮细胞的增殖。
The factors that stimulate kidney growth in K+-deficient animals are unknown. Cultures of renal epithelial cells (BSC-1 line) were used to study this phenomenon because their growth is accelerated in medium containing a reduced K+ concentration. We tested the hypothesis that growth induced by low-K+ medium is mediated by factors produced by the cells; i.e., is subject to autocrine control. Low-K+ (3.2 mM) or control (5.4 mM) medium was conditioned by placing it on confluent cultures of BSC-1 cells for 1 h and was then collected. The K+ concentration of the low-K+ conditioned medium was then adjusted to the control value by addition of KCl. This conditioned medium stimulated growth of fresh cultures of cells to the same extent as did unconditioned low-K+ medium. The appearance of growth-promoting activity was maximal at a K+ concentration of 3.2 mM during conditioning of the medium. Low-K+ conditioned medium, corrected to a K+ concentration of 5.4 mM, required 6 h to commit cells to enhanced proliferation. Growth-stimulating activity in low-K+ conditioned medium was antagonized by a purified growth inhibitor produced by the cells. These observations are consistent with the hypothesis that autocrine products with opposite effects on growth can regulate proliferation of renal epithelial cells.