DR7 AND DQ2 ARE POSITIVELY ASSOCIATED WITH IMMUNOGLOBULIN-E RESPONSE TO THE MAIN ANTIGEN OF OLIVE POLLEN (OLE E I) IN ALLERGIC PATIENTS
DR7 AND DQ2 ARE POSITIVELY ASSOCIATED WITH IMMUNOGLOBULIN-E RESPONSE TO THE MAIN ANTIGEN OF OLIVE POLLEN (OLE E I) IN ALLERGIC PATIENTS
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DOI：
10.1016/0198-8859(93)90557-h
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发表时间：
1993-12-01
期刊：
影响因子：
2.7
通讯作者：
LAHOZ, C
中科院分区：
文献类型：
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作者：
CARDABA, B;VILCHES, C;LAHOZ, C
我们研究了人类白细胞抗原II类单倍型和等位基因之间的关系，以及过敏患者对高纯度变应原Ole E I的IgE抗体反应。Ole e I是主要生长在地中海的橄榄树花粉的主要抗原。采用双抗体放射免疫法对40例抗Ole E I特异性IgE抗体阳性的季节性变态反应性花粉症患者进行了基因组DNA分型。采用聚合酶链式反应-单链构象多态性和限制性片段长度多态性分析方法对HLA-DRB和-DQB基因座进行分析。并与179名健康无关个体的表型频率进行比较。DR7型(PF=67.5%vs31.3%，P-c=0.0023)和DQ2型(Pf=90.0%vs48.0%，Pc=0.0003)的表型频率显著增加，提示DRB1*0701/2、DQB1*0201等位基因与OLE抗体应答有关，这是首次发现HLADQ基因与过敏反应阳性有关。
We have studied the relationship between HLA class II haplotypes and alleles, and the IgE antibody response to a highly purified allergen, Ole e I, in allergic patients. Ole e I, is the major antigen from the pollen of olive tree that grows mainly in the Mediterranean. Genomic DNA typing was performed in 40 unrelated patients with seasonal allergic pollenosis who had specific IgE antibodies against Ole e I, detected by double-antibody radioimmunoassay. HLA-DRB and -DQB loci were analyzed by PCR-SSO and RFLP. Phenotypic frequencies were compared with those of 179 healthy unrelated individuals. Significant increases in the phenotypic frequencies of DR7 (pf = 67.5% vs 31.3% in the control population, P-c = 0.0023) and DQ2 (pf = 90.0% vs 48.0%, P-c = 0.0003) were found, indicating an association between DRB1*0701/2, DQB1*0201 alleles and the IgE antibody response to Ole e I. This is the first time that the HLA-DQ gene has been associated with a positive allergic response.