An amino acid duplication/insertion in the Bm126 gene of Bombyx mori nucleopolyhedrovirus alters viral gene expression as shown by differential gene expression analysis
An amino acid duplication/insertion in the Bm126 gene of Bombyx mori nucleopolyhedrovirus alters viral gene expression as shown by differential gene expression analysis
复制标题
差异基因表达分析显示，家蚕核多角体病毒 Bm126 基因中的氨基酸重复/插入改变了病毒基因表达
DOI：
10.1007/s00705-018-04144-2
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发表时间：
2019-03-01
影响因子：
2.7
通讯作者：
Hao, Bifang
中科院分区：
文献类型：
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作者：
Huang, Jinshan;Li, Jingfeng;Hao, Bifang
家蚕核型多角体病毒（BmNPV）的开放阅读框126（Bm 126）基因是病毒复制所必需的非编码基因，在我国已鉴定出该基因的两个亚型。Bm 126-SX亚型编码一种相对于Bm 126-GD亚型具有简单氨基酸重复/插入的蛋白质;然而，已经观察到携带这些变体基因的病毒的细胞病变效应和感染性的显著差异。为了阐明这些差异的原因，差异基因表达分析进行了感染的早期阶段与病毒窝藏Bm 126的变体。在感染后6、12和24 h，分别观察到vGD 126样品相对于对照样品（vSX 126）的103、209和313个宿主基因以及9、44和67个病毒基因的差异表达。这些结果表明，Bm 126中的重复/插入改变了病毒的表达模式。发现差异表达的宿主基因与核糖体、剪接体和蛋白酶体途径有关，并确定了参与信号转导的几个因子。通过qPCR证实这些病毒和宿主基因的差异表达。本研究表明Bm 126基因中的氨基酸重复/插入具有与病毒基因表达调控相关的生物学功能，并为进一步表征Bm 126基因功能奠定了基础。
Open reading frame (ORF) 126 (Bm126) of Bombyx mori nucleopolyhedrovirus (BmNPV) is not essential for viral replication, and two subtypes of this gene have been identified in China. The Bm126-SX subtype encodes a protein with a simple amino acid duplication/insertion relative to the Bm126-GD subtype; however, significant differences in the cytopathic effect and infectivity of viruses carrying these variant genes have been observed. To elucidate the cause of these differences, differential gene expression analysis was performed at the early stage of infection with viruses harbouring variants of Bm126. Differential expression was observed for 103, 209, and 313 host genes and 9, 44, and 67 viral genes in vGD126 samples relative to the control samples (vSX126) at 6, 12, and 24 h postinfection, respectively. These results indicated that the duplication/insertion in Bm126 altered the viral expression pattern. The differentially expressed host genes were found to be related to ribosome, spliceosome, and proteasome pathways, and several factors involved in signal transduction were also identified. The differential expression of these viral and host genes was confirmed by qPCR. This study indicates that the amino acid duplication/insertion in the Bm126 gene has a biological function related to the regulation of viral gene expression and serves as a basis for further characterization of Bm126 gene function.