ACTIVITIES OF LIVER MITOCHONDRIAL AND PEROXISOMAL FATTY-ACID OXIDATION ENZYMES IN RATS FED TRANS FAT
ACTIVITIES OF LIVER MITOCHONDRIAL AND PEROXISOMAL FATTY-ACID OXIDATION ENZYMES IN RATS FED TRANS FAT
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DOI：
10.1007/bf02534867
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发表时间：
1987-01-01
期刊：
影响因子：
1.9
通讯作者：
YAMAMOTO, I
中科院分区：
文献类型：
--
作者：
IDE, T;WATANABE, M;YAMAMOTO, I
本文观察了反式脂肪酸对不同品系大鼠肝线粒体和过氧化物酶体脂肪酸氧化酶活性的影响。Wistar和Sprague-Dawley大鼠分别以橄榄油或部分氢化玉米油作为顺式或反式十八碳烯酸的来源，饲喂30天后，以顺式和反式-9-十八碳烯酸为底物测定的线粒体和过氧化物酶体β-氧化的各种酶活性几乎没有饮食脂肪依赖性变化。在Fischer 344大鼠中，喂食transfat 15个月，除了肉毒碱酰基转移酶外，仅适度增加了β-氧化的各种酶。以反式-9-十八碳烯酸为底物测定的线粒体生酮速率和肉毒碱酰基转移酶活性约为反式-9-十八碳烯酸的一半。过氧化物酶体氧化反式-9-十八烯酰-CoA的速率与顺式对应物相当。从这项研究和以前的研究中得出结论，饮食脂肪酸的几何形状的差异在调节肝脏脂肪酸氧化系统方面只有很小的影响，尽管在无细胞制剂和灌注肝脏中顺式和反式脂肪酸的代谢行为存在显著差异。
The effect oftransfat on the activities of liver mitochondrial and peroxisomal fatty acid oxidation enzymes was examined in various strains of rats. When Wistar and Sprague-Dawley rats were fed for 30 days diets containing either olive oil or partially hydrogenated corn oil as a source ofcis-ortrans-octadecenoate, respectively, the activities of various enzymes of mitochondrial and peroxisomal β-oxidation measured withcis- andtrans-9-octadecenoic acid as substratese showed little dietary fatdependent change. In Fischer 344 rats, feedingtransfat for 15 mo increased only moderately various enzymes of β-oxidation except for carnitine acyltransferase. The rate of mitochondrial ketogenesis and the activity of carnitine acyltransferase measured withtrans-9-octadecenoic acid as a substrate were about half those with thecis-counterpart. Peroxisomes oxidizedtrans-9-octadecenoyl-CoA at a rate comparable to thecis-counterpart. It was concluded from this study and previous ones that the difference in the geometry of dietary fatty acid had only a marginal effect in modulating the hepatic fatty acid oxidation system, in spite of marked differences in the metabolic behavior ofcis-andtransfatty acid in cell-free preparations and perfused liver.