Regulation of Arabidopsis Embryo and Endosperm Development by the Polypeptide Signaling Molecule CLE8
Regulation of Arabidopsis Embryo and Endosperm Development by the Polypeptide Signaling Molecule CLE8
复制标题
DOI：
10.1105/tpc.111.094839
复制
发表时间：
2012-03-01
期刊：
影响因子：
11.6
通讯作者：
Fletcher, Jennifer C.
中科院分区：
文献类型：
--
作者：
Fiume, Elisa;Fletcher, Jennifer C.
植物种子是人类的主要营养来源，也是胚胎发育和传播的重要单位。为了确保种子的正确形成，胚、胚乳和母本种子成分之间必须达到良好的空间和时间协调。然而，指导这些组织同步发育的细胞间信号传导途径知之甚少。在这里，我们表明，拟南芥肽配体CLAVATA 3/胚周围区域-胚乳8（CLE 8）是专门在年轻的胚胎和胚乳中表达，它的行为细胞和非细胞自主调节基础胚胎细胞分裂模式，胚乳增殖，胚乳分化的时间。CLE 8正调控转录因子基因WUSCHEL样HOMEOBOX 8（WOX 8）的表达，并且CLE 8和WOX 8一起形成促进种子生长和总体种子大小的信号传导模块。这些结果表明，种子发育是由分泌的肽配体协调的，所述肽配体在胚胎和胚乳中协调细胞图案化和增殖中起关键的早期作用。
The plant seed is a major nutritional source for humans as well as an essential embryo development and dispersal unit. To ensure proper seed formation, fine spatial and temporal coordination between the embryo, endosperm, and maternal seed components must be achieved. However, the intercellular signaling pathways that direct the synchronous development of these tissues are poorly understood. Here we show that the Arabidopsis thaliana peptide ligand CLAVATA3/EMBRYO SURROUNDING REGION-RELATED8 (CLE8) is exclusively expressed in young embryos and endosperm, and that it acts cell and noncell autonomously to regulate basal embryo cell division patterns, endosperm proliferation, and the timing of endosperm differentiation. CLE8 positively regulates expression of the transcription factor gene WUSCHEL-LIKE HOMEOBOX8 (WOX8), and together CLE8 and WOX8 form a signaling module that promotes seed growth and overall seed size. These results demonstrate that seed development is coordinated by a secreted peptide ligand that plays a key early role in orchestrating cell patterning and proliferation in the embryo and endosperm.