EPIDERMAL GROWTH-FACTOR AND MULTIPLICATION OF CULTURED HUMAN EPIDERMAL KERATINOCYTES
EPIDERMAL GROWTH-FACTOR AND MULTIPLICATION OF CULTURED HUMAN EPIDERMAL KERATINOCYTES
复制标题
DOI：
10.1038/265421a0
复制
发表时间：
1977-01-01
期刊：
影响因子：
64.8
通讯作者：
GREEN, H
中科院分区：
文献类型：
--
作者：
RHEINWALD, JG;GREEN, H
通过向培养基中添加表皮生长因子（EGF）（一种多肽有丝分裂原），新生儿表皮细胞的培养寿命从50代增加到150代。EGF似乎通过将细胞维持在远离终端分化的状态来延缓细胞的衰老。这种效应是通过细胞在继代培养中存活和产生新菌落的更大能力来揭示的，但不一定是通过增加的生长速度来揭示的。
The culture lifetime of epidermal cells of newborn humans is increased from 50 to 150 generations by adding to the medium epidermal growth factor [EGF], a polypeptide mitogen. EGF seems to delay senescence of the cells by maintaining them in a state further removed from terminal differentiation. This effect is revealed by a greater ability of the cells to survive subculture and initiate new colonies, but not necessarily by an increased growth rate.