A Quorum-Quenching Approach To Investigate the Conservation of Quorum-Sensing-Regulated Functions within the Burkholderia cepacia Complex
A Quorum-Quenching Approach To Investigate the Conservation of Quorum-Sensing-Regulated Functions within the Burkholderia cepacia Complex
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DOI：
10.1128/aem.72.2.1579-1587.2006
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发表时间：
2006-02
影响因子：
4.4
通讯作者：
J. Wopperer;Silvia T. Cardona;B. Huber;C. A. Jacobi;M. Valvano;L. Eberl
中科院分区：
文献类型：
--
作者：
J. Wopperer;Silvia T. Cardona;B. Huber;C. A. Jacobi;M. Valvano;L. Eberl
摘要过去几年的分类学研究表明，洋葱伯克霍尔德菌复合体是一个异质的B.类洋葱生物，由至少9种。B。洋葱复合体菌株在自然界中广泛分布，并已用于生物防治、生物修复和植物生长促进目的。同时，B。洋葱复合体菌株已经成为人类，特别是患有囊性纤维化的人类的重要机会致病菌。全是B。迄今为止研究的洋葱复合体物种使用群体感应（QS）系统，该系统依赖于N-酰基高丝氨酸内酯（阿勒）信号分子以群体密度依赖性方式表达某些功能，包括产生细胞外蛋白酶、群集运动性、生物膜形成和致病性。在这项研究中，我们构建了一个广泛的宿主范围的质粒，允许异源表达芽孢杆菌菌株240 B1 AiiA内酯酶，水解各种阿勒信号分子的内酯环，在所有描述的B。洋葱复合种我们表明AiiA的表达消除或大大减少了所有测试的B的培养上清液中阿勒分子的积累。洋葱复合体菌株。野生型和转基因菌株的表型表征表明，蛋白酶的生产，群集运动，生物膜的形成，秀丽隐杆线虫的杀伤效率调节阿勒在绝大多数菌株的调查。
ABSTRACT Taxonomic studies of the past few years have shown that the Burkholderia cepacia complex, a heterogeneous group of B. cepacia-like organisms, consists of at least nine species. B. cepacia complex strains are ubiquitously distributed in nature and have been used for biocontrol, bioremediation, and plant growth promotion purposes. At the same time, B. cepacia complex strains have emerged as important opportunistic pathogens of humans, particularly those with cystic fibrosis. All B. cepacia complex species investigated thus far use quorum-sensing (QS) systems that rely on N-acylhomoserine lactone (AHL) signal molecules to express certain functions, including the production of extracellular proteases, swarming motility, biofilm formation, and pathogenicity, in a population-density-dependent manner. In this study we constructed a broad-host-range plasmid that allowed the heterologous expression of the Bacillus sp. strain 240B1 AiiA lactonase, which hydrolyzes the lactone ring of various AHL signal molecules, in all described B. cepacia complex species. We show that expression of AiiA abolished or greatly reduced the accumulation of AHL molecules in the culture supernatants of all tested B. cepacia complex strains. Phenotypic characterization of wild-type and transgenic strains revealed that protease production, swarming motility, biofilm formation, and Caenorhabditis elegans killing efficiency was regulated by AHL in the large majority of strains investigated.