Electrospun poly(L-lactide-co-caprolactone)-collagen-chitosan vascular graft in a canine femoral artery model
Electrospun poly(L-lactide-co-caprolactone)-collagen-chitosan vascular graft in a canine femoral artery model
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犬股动脉模型中的静电纺丝聚（L-丙交酯-己内酯）-胶原-壳聚糖血管移植物
DOI：
10.1039/c5tb00599j
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发表时间：
2015
影响因子：
7
通讯作者：
Mo Xiumei
中科院分区：
文献类型：
--
作者：
Wu Tong;Jiang Bojie;Wang Yuanfei;Yin Anlin;Huang Chen;Wang Sheng;Mo Xiumei
本研究采用聚l -乳酸-co-己内酯-胶原-壳聚糖（P(la - cl) -COL-CS）复合材料静电纺丝接枝。根据力学性能、生物降解性和体外细胞相容性试验结果，确定P（LLA-CL）与COL/CS的最佳重量比为3:1。在犬股动脉模型中进一步进行了体内研究。结果表明，与基于P（LLA-CL）的移植物相比，3∶1的移植物具有良好的结构完整性，更高的通畅率，更好的内皮细胞（EC）和平滑肌细胞（SMC）生长，以及更高水平的血管生成相关线索的基因和蛋白表达。研究结果证实，胶原蛋白和壳聚糖等天然材料的加入可有效促进组织工程血管的内皮化、SMC向中膜的浸润和血管重构。
Poly(L-lactide-co-caprolactone)–collagen–chitosan (P(LLA-CL)–COL–CS) composite grafts were electrospun in this study. Based on the test results for mechanical properties, biodegradability and in vitro cellular compatibility, the optimal weight ratio of P(LLA-CL) to COL/CS was set as 3 : 1. In vivo study was further performed in a canine femoral artery model. The results showed that the 3 : 1 grafts possessed excellent structural integrity, higher patency rate, better endothelial cell (EC) and smooth muscle cells (SMC) growth, as well as higher levels of gene and protein expression of angiogenesis-related cues than those of grafts based on P(LLA-CL). The findings confirmed that the addition of natural materials, such as collagen and chitosan, could effectively improve endothelialization, SMC incursion into the tunica media, and vascular remodeling for tissue engineering.