Synthesis and interferon-gamma controlled release of pteridines during activation of human peripheral blood mononuclear cells.
Synthesis and interferon-gamma controlled release of pteridines during activation of human peripheral blood mononuclear cells.
复制标题
人外周血单核细胞激活过程中蝶啶的合成和干扰素-γ控制释放。
DOI：
10.1016/0006-291x(85)91036-8
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
I. Ziegler
中科院分区：
文献类型：
--
作者：
I. Ziegler
经碘氧化后用HPLC测定，凝集素刺激人外周血单个核细胞可引起新蝶呤、生物蝶呤、6-羟甲基蝶呤和6-甲酰基蝶呤的增加。72小时后，与静止细胞相比，翼啶的水平达到5-10倍。在接下来的24小时内，水平下降到初始值。蝶呤比例的变化表明，在淋巴细胞活化过程中，由三磷酸二氢蝶呤合成四氢蝶呤受到控制。细胞内新蝶呤和生物蝶呤的释放，但6-羟甲基蝶呤及其醛的释放不受干扰素-γ的控制。
Lectin stimulation of human peripheral blood mononuclear cells causes an increase in neopterin, biopterin, 6-hydroxymethylpterin and 6-formylpterin, as was determined by HPLC after iodine oxidation of the acid extract. After 72 h, pteridines peak at levels 5–10 fold as compared to resting cells. Levels decline to initial values during the following 24 h. Changes in pteridine proportions indicate that the synthesis of tetrahydrobiopterin proceeding from dihydroneopterin triphosphate is controlled during the process of lymphocyte activation. The release of both cellular neopterin and biopterin, but not of 6-hydroxymethylpterin and its aldehyde, is controlled by interferon-γ.