An Overview of PulseNet USA Databases
An Overview of PulseNet USA Databases
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DOI：
10.1089/fpd.2019.2637
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发表时间：
2019-05-08
影响因子：
2.8
通讯作者：
Gerner-Smidt, Peter
中科院分区：
文献类型：
--
作者：
Tolar, Beth;Joseph, Lavin A.;Gerner-Smidt, Peter
PulseNet USA是美国食源性疾病的分子监测网络。该网络由州和地方公共卫生实验室以及食品监管机构组成，它们遵循PulseNet的标准化协议进行脉冲场凝胶电泳（PFGE）和全基因组测序（WGS），并使用标准化软件分析结果。原始序列被上传到国家生物技术信息中心的GenomeTrakr或PulseNet生物项目。PFGE模式和分析的序列数据与相关的人口统计学数据一起真实的实时上传到疾病控制和预防中心管理的PulseNet国家数据库。PulseNet数据库是微生物特异性的，为分离株相关的分子和人口统计学数据提供了一个中央存储位置。序列在数据库中进行比较，从而有助于快速检测可能代表广泛爆发的食源性疾病集群。WGS基因分型数据，例如抗生素耐药性和毒力谱，也被真实的实时上传到PulseNet数据库，以改善食品安全监测活动。
PulseNet USA is the molecular surveillance network for foodborne disease in the United States. The network consists of state and local public health laboratories, as well as food regulatory agencies, that follow PulseNet's standardized protocols to perform pulsed-field gel electrophoresis (PFGE) and whole genome sequencing (WGS) and analyze the results using standardized software. The raw sequences are uploaded to the GenomeTrakr or PulseNet bioprojects at the National Center for Biotechnology Information. The PFGE patterns and analyzed sequence data are uploaded in real time with associated demographic data to the PulseNet national databases managed at the Centers for Disease Control and Prevention. The PulseNet databases are organism specific and provide a central storage location for molecular and demographic data related to an isolate. Sequences are compared in the databases, thereby facilitating the rapid detection of clusters of foodborne diseases that may represent widespread outbreaks. WGS genotyping data, for example, antibiotic resistance and virulence profiles, are also uploaded in real time to the PulseNet databases to improve food safety surveillance activities.