Immunogenicity of recombinant LT-B delivered orally to humans in transgenic corn.
Immunogenicity of recombinant LT-B delivered orally to humans in transgenic corn.
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DOI：
10.1016/j.vaccine.2004.01.073
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发表时间：
2004-10
期刊：
影响因子：
5.5
通讯作者：
C. Tacket;M. Pasetti;R. Edelman;J. Howard;S. Streatfield
中科院分区：
文献类型：
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作者：
C. Tacket;M. Pasetti;R. Edelman;J. Howard;S. Streatfield
以前的临床研究已经证明了使用可食用转基因植物作为新的口服疫苗提供保护性抗原的可行性。转基因玉米在这方面特别有吸引力，因为重组抗原是稳定和均匀的，并且玉米可以在不破坏克隆抗原的情况下制成几种可食用的形式。将表达1mg未加缓冲剂的大肠杆菌LT-B的转基因玉米分三次喂给成年志愿者，每次2.1g植物材料。9名志愿者中有7名（78%）在接种疫苗后血清IgG抗lt和特异性抗体分泌细胞数量均上升。9名志愿者中有4名（44%）也患有大便IgA。转基因植物是一种新的口服疫苗抗原载体。
Previous clinical studies have demonstrated the feasibility of using edible transgenic plants to deliver protective antigens as new oral vaccines. Transgenic corn is particularly attractive for this purpose since the recombinant antigen is stable and homogeneous, and corn can be formulated in several edible forms without destroying the cloned antigen. Transgenic corn expressing 1mg of LT-B of Escherichia coli without buffer was fed to adult volunteers in three doses, each consisting of 2.1g of plant material. Seven (78%) of nine volunteers developed rises in both serum IgG anti-LT and numbers of specific antibody secreting cells after vaccination. Four (44%) of nine volunteers also developed stool IgA. Transgenic plants represent a new vector for oral vaccine antigens.