Evaluation of the immune response following a short oral vaccination schedule with hepatitis B antigen encapsulated into alginate-coated chitosan nanoparticles
Evaluation of the immune response following a short oral vaccination schedule with hepatitis B antigen encapsulated into alginate-coated chitosan nanoparticles
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DOI：
10.1016/j.ejps.2007.08.005
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发表时间：
2007-12-01
影响因子：
4.6
通讯作者：
Cordeiro-da-Silva, Anabela
中科院分区：
文献类型：
--
作者：
Borges, Olga;Tavares, Joana;Cordeiro-da-Silva, Anabela
本研究的目的是评估重组B型肝炎疫苗，封装在海藻酸盐包被的壳聚糖纳米颗粒，诱导口服疫苗后的局部和全身免疫应答的能力。将抗原单独或与免疫增强剂、含有免疫刺激性CpG基序的合成寡脱氧核苷酸（CpG ODN）作为佐剂组合施用，并与藻酸盐包被的壳聚糖纳米颗粒结合或不结合。两次免疫后，组I（与纳米颗粒相关的HBsAg）和组VI（均与纳米颗粒相关的HBsAg和CpG）显示增强的免疫应答。两组均显示CD 4+和CD 8 + T淋巴细胞中CD 69表达值显著较高，而B淋巴细胞中该标记物的值较低。此外，最强的增殖反应的脾细胞，离体刺激伴刀豆球蛋白A，在相同的组中观察到。尽管组内存在无应答小鼠，但仅I组和VI组小鼠引起血清（IgG）和肠道冲洗液（sIgA）中检测到抗HBsAg抗体的产生。结果表明，壳聚糖纳米粒有可能被用作口服疫苗的重组B表面抗原的传递系统。(C)2007 Elsevier B.V.保留所有权利。
The purpose of this work was to assess the ability of recombinant hepatitis B vaccine, encapsulated in alginate-coated chitosan nanoparticles, to induce local and systemic immune responses following oral vaccination. The antigen was administered either alone or in combination with the immunopotentiator, synthetic oligodeoxynucleotide containing immunostimulatory CpG motif (CpG ODN) as adjuvant, and associated or not with the alginate-coated chitosan nanoparticles. After two immunizations the group I (HBsAg associated with nanoparticles) and the group VI (HBsAg and CpG, both associated with nanoparticles) showed enhanced immune responses. Both groups showed significant higher values of the CD69 expression in CD4+ and CD8+ T-lymphocytes and lower values of this marker in B lymphocytes. Moreover, a strongest proliferative response of the splenocytes, ex vivo stimulated with concanavalin A, was observed in the same groups. Although with a presence of non-responder mice within the groups, only mice of the groups I and VI elicited the generation of anti-HBsAg antibodies detected in serum (IgG) and in the intestinal washings (sIgA). The results demonstrated that coated chitosan nanoparticles might have potential for being used as a deliver system for oral vaccination with the recombinant hepatitis B surface antigen. (C) 2007 Elsevier B.V. All rights reserved.