Molecular characterization of bacterial diversity from British Columbia forest soils subjected to disturbance
Molecular characterization of bacterial diversity from British Columbia forest soils subjected to disturbance
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DOI：
10.1139/w02-059
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发表时间：
2002-07-01
影响因子：
2.8
通讯作者：
Davies, J
中科院分区：
文献类型：
--
作者：
Axelrood, PE;Chow, ML;Davies, J
通过对克隆的16S rRNA基因片段（16S克隆）进行DNA序列分析，对森林土壤中的细菌进行了特征描述。从英属哥伦比亚省林业部长期土壤生产力（LTSP）试验点采集了地表有机物和矿质土壤样本，分别在冬季和夏季从两种干扰处理方式中采集：整树采伐且无土壤压实（N区）以及整树采伐加上完全去除地表有机物且重度土壤压实（S区）。系统发育分析显示，580个16S克隆中有87%被归类为变形菌门、放线菌门、酸杆菌门、疣微菌门、芽孢杆菌/梭菌属群、噬纤维菌 - 屈挠杆菌 - 拟杆菌群、绿色非硫细菌、浮霉菌以及候选分类群TM6和OP10。75个16S克隆无法归类到已知的细菌分类群中，5个16S克隆与叶绿体DNA有关。变形菌门的成员占克隆文库的46%。与S区相比，N区中属于γ - 变形菌纲的16S克隆比例更高。从三个LTSP试验点的N区和S区的矿质土壤样本中，对用假单胞菌特异性引物扩增并克隆的16S rRNA基因片段（Ps克隆）进行了检测。与S区的Ps克隆相比，N区测序的Ps克隆中含有假单胞菌16S rRNA基因片段的比例显著更高。
Bacteria from forest soils were characterized by DNA sequence analysis of cloned 16S rRNA gene fragments (16S clones). Surface organic matter and mineral soil samples from a British Columbia Ministry of Forests Long-Term Soil Productivity (LTSP) installation were collected during winter and summer from two disturbance treatments: whole-tree harvesting with no soil compaction (plot N) and whole-tree harvesting plus complete surface organic matter removal with heavy soil compaction (plot S). Phylogenetic analyses revealed that 87% of 580 16S clones were classified as Proteobacteria, Actinobacteria, Acidobacterium, Verrucomicrobia, Bacillus/Clostridium group, Cytophaga-Flexibacter-Bacteroides group, green nonsulfur bacteria, Planctomyces, and candidate divisions TM6 and OP10. Seventy-five 16S clones could not be classified into known bacterial divisions, and five 16S clones were related to chloroplast DNA, Members of Proteobacteria represented 46% of the clone library. A higher proportion of 16S clones affiliated with gamma-Proteobacteria were from plot N compared with plot S. 16S rRNA gene fragments amplified with Pseudomonas-specific primers and cloned (Ps clones) were examined from mineral-soil samples from plots N and S from three LTSP installations. A significantly greater proportion of sequenced Ps clones from plot N contained Pseudomonas 16S rRNA gene fragments compared with Ps clones from plot S.