CCL3 serves as a potential plasma biomarker in knee degeneration (osteoarthritis).
CCL3 serves as a potential plasma biomarker in knee degeneration (osteoarthritis).
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DOI：
10.1016/j.joca.2015.04.002
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发表时间：
2015-08
影响因子：
7
通讯作者：
Xiaoyi Zhao;Zi‐bo Yang;Ziji Zhang;Zhiqi Zhang;Yan Kang;Guangxin Huang;S. Wang;H. Huang;W. Liao
中科院分区：
文献类型：
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作者：
Xiaoyi Zhao;Zi‐bo Yang;Ziji Zhang;Zhiqi Zhang;Yan Kang;Guangxin Huang;S. Wang;H. Huang;W. Liao
目的探讨血浆趋化因子检测x线前膝关节退变及其程度（负担）的能力。方法181例患者（对照组75例，x - kd前期患者47例，X-KOA患者50例）分为3个亚组。术前x - kd患者的关节软骨损失在关节镜检查时根据ICRS分类进行评分，或在MRI上记录软骨WORMS。X-KOA的严重程度通过膝关节前后x光片采用kelgren - lawrence分级进行分级。采用Luminex微球悬浮阵列（microbead-based suspension array， SA）定量检测血浆中炎症因子和趋化因子的浓度，并采用酶联免疫吸附试验（ELISA）交叉验证。结果血浆sccl3对x - kd前期患者的鉴别能力最高，AUC为0.799。在截断值为0.168 pg/ml时，敏感性为70.21%，特异性为96.00%，阳性预测值为91.67%，阴性预测值为83.72%。在定义疾病负担方面，血浆抵抗素、il - 6、il - 8、CCL3和CCL4水平与x线诊断的膝关节OA的严重程度有显著相关性。此外，与预x - kd患者（KL分级0-I）相比，严重膝关节OA患者（KL分级IV）血浆中IL6、IL8、CCL3和CCL4水平显著升高。结论我们首先发现血浆CCL3可能是膝关节OA的潜在血清生物标志物，具有检测x线前确定的变化和膝关节损伤严重程度的能力。
ObjectiveTo explore the ability of chemokines in plasma to detect the presence of pre-X-rays defined knee degeneration and the extent (burden).MethodsA total of 181 subjects (75 control subjects, 47 pre-X-KD patients and 50 X-KOA patients) were included and subdivided into three subgroups. Articular cartilage loss in pre-X-KD patients were scored on the basis of the ICRS classification during the arthroscopy or documented on MRI with chondral WORMS. The severity of X-KOA was graded using the Kellgren–Lawrence classification through the posterior–anterior knee X-rays. The concentrations of the inflammatory cytokines and chemokines in plasma were quantified using Luminex microbead-based suspension array (SA) and were cross-validated by enzyme-linked immunosorbent assay (ELISA).ResultsCCL3 in plasma showed the highest ability to discriminate pre-X-KD patients from the controls with an AUC of 0.799. At a cutoff value of 0.168 pg/ml, the sensitivity was 70.21%, the specificity was 96.00%, the positive predictive value was 91.67% and the negative predictive value was 83.72%. As to define disease burden, the plasma levels of resistin, IL6, IL8, CCL3 and CCL4 showed significant association with the severity of X-rays defined knee OA, with regard to the KL classification. Moreover, significant elevation of IL6, IL8, CCL3 and CCL4 levels in plasma were observed in severe knee OA patients (KL grade IV) compared with those with pre-X-KD (KL grade 0–I).ConclusionWe firstly showed that the plasma CCL3 could be potential serum biomarker for knee OA with the capacity to detect pre-X-rays defined changes and stage the severity of damage in knee.