E-selectin targeting to visualize tumors in vivo
E-selectin targeting to visualize tumors in vivo
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DOI：
10.1002/cmmi.367
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发表时间：
2010-03-01
影响因子：
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通讯作者：
Seno, Masaharu
中科院分区：
文献类型：
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作者：
Hirai, Masahiko;Hiramatsu, Yoshie;Seno, Masaharu
血管生成因子通常诱导肿瘤血管内皮细胞表达E-选择素。在这项研究中，我们采用了抗E-选择素单克隆抗体在体内靶向肿瘤，并评估了光学成像试剂可视化肿瘤区域。将抗E-选择素抗体偶联在脂质体的表面上，脂质体包封近红外荧光物质Cy 3或Cy5.5。脂质体有效地识别人脐静脉内皮细胞，只有当E-选择素诱导的血管生成因子，如TNF-α在体外。当移植到小鼠中时，包封到与抗E-选择素抗体缀合的脂质体中的Cy5.5成功地使埃利希腹水肿瘤细胞可视化。因此，发现用含有近红外荧光染料的脂质体靶向E-选择素在体内可视化肿瘤中是有效的。这种策略应该是非常有用的方法，以确定前哨淋巴结和血管生成肿瘤，以及用于药物输送到肿瘤细胞。版权所有（C）2010约翰威利父子有限公司
Generally angiogenic factors induce the expression of E-selectin in vascular endothelial cells in the tumors. In this study, we employed an anti-E-selectin monoclonal antibody to target tumors in vivo and evaluated an optical imaging reagent to visualize tumor regions. The anti-E-selectin antibody was conjugated on the surface of liposomes, which encapsulated the near-infrared fluorescent substances Cy3 or Cy5.5. The liposomes efficiently recognized human umbilical vein endothelial cells only when E-selectin was induced by angiogenic factors such as TNF-alpha in vitro. Cy5.5 encapsulated into liposomes that were conjugated with an anti-E-selectin antibody successfully visualized Ehrlich ascites tumor cells when transplanted into mice. Thus, E-selectin targeting with liposomes containing a near-infrared fluorescent dye was found effective in visualizing tumors in vivo. This strategy should be extremely useful as a method to identify sentinel lymphatic nodes and angiogenic tumors as well as use for drug delivery to tumor cells. Copyright (C) 2010 John Wiley & Sons, Ltd.