Interactions of antibiotics and phagocytes.
Interactions of antibiotics and phagocytes.
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抗生素和吞噬细胞的相互作用。
DOI：
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发表时间：
1983
影响因子：
5.2
通讯作者：
T. Steinberg
中科院分区：
文献类型：
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作者：
W. Hand;N. King;T. Steinberg
由于能够在吞噬细胞内存活的生物体引起的感染的最佳治疗将包括使用穿透吞噬细胞并杀死细胞内生物体的抗菌剂。为了建立介导抗生素-吞噬细胞相互作用的药物和细胞的特征，我们研究了兔肺泡巨噬细胞（AM）、吸烟者和非吸烟者的人AM以及人多形核白细胞（PMN）对放射性标记抗生素的摄取。药物组进入三种类型吞噬细胞的相对数量相似。青霉素G和头孢菌素类抗生素被吞噬细胞吸收较差。脂溶性抗生素，如利福平和氯霉素，被吞噬细胞浓缩几倍（2-5）。乙胺丁醇、红霉素和克林霉素被吞噬细胞浓缩许多倍（5-50）。吸烟者的AM比非吸烟者的AM更贪婪地积累某些抗生素。克林霉素进入吞噬细胞被证明是一个积极的，需要能量的过程，介导的核苷转运系统。PMN摄取微生物颗粒刺激克林霉素和核苷（腺苷）转运到细胞内。
Optimal therapy of infections due to organisms capable of surviving within phagocytes would include use of antimicrobials that penetrate phagocytic cells and inactivate intracellular organisms. To establish those characteristics of drug and cell that mediate the antibiotic-phagocyte interaction, we have studied the uptake of radiolabelled antibiotics by rabbit alveolar macrophages (AM), human AM from smokers and non-smokers, and human polymorphonuclear leukocytes (PMN). Relative entries of drug groups into the three types of phagocytic cells were similar. Penicillin G and cephalosporin antibiotics were taken up poorly by phagocytes. Lipid-soluble antibiotics, such as rifampicin and chloramphenicol, were concentrated several-fold (2-5) by phagocytes. Ethambutol, erythromycin and clindamycin were concentrated many-fold (5-50) by phagocytic cells. Human AM of smokers accumulated certain antibiotics more avidly than AM of non-smokers. Clindamycin entry into phagocytes was shown to be an active, energy-requiring process, mediated by the nucleoside transport system. Ingestion of microbial particles by PMN stimulated transport of both clindamycin and nucleoside (adenosine) into the cell.