Protocol to create a murine subcutaneous air pouch for the study of monosodium urate crystal-induced gout.
Protocol to create a murine subcutaneous air pouch for the study of monosodium urate crystal-induced gout.
复制标题
创建小鼠皮下气囊用于研究尿酸钠晶体诱发的痛风的方案。
DOI：
10.1016/j.xpro.2024.102888
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Dorfleutner,Andrea
中科院分区：
文献类型：
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作者：
Devi,Savita;Stehlik,Christian;Dorfleutner,Andrea
尿酸单钠（MSU）晶体沉积在关节和囊组织引起急性关节炎症，这是痛风的标志。在这里，我们描述了在小鼠背部建立皮下气囊的必要步骤，该气囊类似于具有滑膜衬里样膜的囊样空间。然后，我们详细介绍了将MSU晶体注射到这个囊袋中，这引起了局部炎症反应，让人想起痛风和方法来量化炎症反应。（2023），1de Almeida et al.（2022），2and Ratsimandresy et al.（2017年）.3
Monosodium urate (MSU) crystal deposition in articular joints and bursal tissue causes acute joint inflammation, which is a hallmark of gout. Here, we describe the steps necessary to create a subcutaneous air pouch on the back of mice that resembles this bursa-like space with a synovial lining-like membrane. We then detail the injection of MSU crystals into this pouch, which induces a localized inflammatory response reminiscent of gout and approaches to quantify the inflammatory response.For complete details on the use and execution of this protocol, please refer to Devi et al. (2023),1de Almeida et al. (2022),2and Ratsimandresy et al. (2017).3