THE STRUCTURE OF POLIOVIRUS REPLICATIVE FORM
THE STRUCTURE OF POLIOVIRUS REPLICATIVE FORM
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DOI：
10.1093/nar/8.6.1217
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发表时间：
1980-01-01
影响因子：
14.9
通讯作者：
WIMMER, E
中科院分区：
文献类型：
--
作者：
LARSEN, GR;DORNER, AJ;WIMMER, E
脊髓灰质炎复制型（RF）的结构已通过3′末端标记和多核苷酸磷酸化酶的使用进行了研究，现在允许RF结构的完整复合物。所提供的证据表明，负链的3′端序列与脊髓灰质炎RNA的5′端完全互补。这表明脊髓灰质炎RNA的5′末端U是遗传编码的。其他数据显示，除了基因编码的聚（A）束的正链RF，单链聚（A）尾伸出超过双链RNA。
The structure of polio replicative form (RF) has been investigated by 3′ end labeling and the use of polynucleotide phosphorylase to now allow a complete composite of the RF structure. The evidence presented indicates that the 3′ terminal sequence of the minus strand is an exact complement to the 5′ end of polio RNA. This suggests that the 5′ terminal U of polio RNA is genetically coded. Other data is presented to show that in addition to the genetically coded poly(A) tract of the plus strand in RF, a single-stranded poly(A) tail protrudes beyond the double-stranded RNA.