Effective Enzyme Immobilization onto a Magnetic Chitin Nanofiber Composite
Effective Enzyme Immobilization onto a Magnetic Chitin Nanofiber Composite
复制标题
磁性甲壳素纳米纤维复合材料上有效的酶固定化
DOI：
10.1021/acssuschemeng.8b01150
复制
发表时间：
2018-07-01
影响因子：
8.4
通讯作者：
Mao, Xiangzhao
中科院分区：
文献类型：
--
作者：
Huang, Wen-Can;Wang, Wei;Mao, Xiangzhao
在本研究中，开发了一种新型生物相容性磁性甲壳素纳米纤维复合材料（MCNC）作为酶固定化的载体，并使用胰凝乳蛋白酶（CT）作为模型酶阐明了酶固定化能力。通过 2,2,6,6-四甲基哌啶-1-氧基 (TEMPO) 介导的甲壳素氧化制备甲壳素纳米纤维 (CNF)，然后用磁性纳米颗粒进一步修饰。戊二醛用于交联额外的 CT 分子并将其聚集到 MCNC 上。通过透射电子显微镜和傅里叶变换红外光谱对 CNF 进行了表征。结果表明，CNF 已正确形成，并且固定在 MCNC 上的 CT 分子表现出优异的性能。将复合材料在60℃下加热3小时后，非交联和交联固定化CT分别保留了初始活性的51.6%和70.7%，而游离CT仅保留了初始活性的29.6%。此外，非交联和交联...
In this study, a novel biocompatible magnetic chitin nanofiber composite (MCNC) was developed as a support for enzyme immobilization, and the enzyme-immobilizing ability was elucidated using chymotrypsin (CT) as a model enzyme. Chitin nanofibers (CNFs) were prepared via 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-mediated oxidation of chitin and then further modified with magnetic nanoparticles. Glutaraldehyde was used to cross-link the additional CT molecules and aggregate them onto the MCNCs. The CNFs were characterized by transmission electron microscopy, and Fourier transform infrared spectroscopy. The results showed that the CNFs were properly formed and that the CT molecules immobilized on the MCNCs presented excellent properties. After heating the composites at 60 °C for 3 h, the non-cross-linked and cross-linked immobilized CTs retained 51.6% and 70.7% of the initial activity, respectively, whereas the free CTs retained only 29.6% of the initial activity. In addition, non-cross-linked and cross-l...