Development of T lymphocytes in Xenopus laevis: appearance of the antigen recognized by an anti-thymocyte mouse monoclonal antibody.
Development of T lymphocytes in Xenopus laevis: appearance of the antigen recognized by an anti-thymocyte mouse monoclonal antibody.
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非洲爪蟾 T 淋巴细胞的发育：抗胸腺细胞小鼠单克隆抗体识别抗原的出现。
DOI：
10.1016/0012-1606(86)90203-4
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发表时间：
1986
影响因子：
2.7
通讯作者：
S. Nagata
中科院分区：
文献类型：
--
作者：
S. Nagata
用抗J系青蛙胸腺细胞表面决定簇的单克隆抗体XT-1研究了非洲爪蟾T淋巴细胞的发育。采用免疫荧光的个体发生研究表明，XT-1 mAb识别的决定簇阳性细胞（XT-1+细胞）首先在J株Xenopus的胸腺中被Nieuwkoop和Faber检测到，第48期（受精后7天），然后在脾脏、肝脏和肾脏中被检测到，第52期（受精后20天）。胸腺中XT-1+细胞的数量在第49阶段（受精后10天）迅速增加，并在第52阶段达到成人水平，脾脏、肝脏和肾脏中的数量在第56阶段（受精后40天）达到成人水平。电镜免疫组化结果显示，56期幼虫胸腺内XT-1+细胞多为典型的小淋巴细胞（直径4-7 μm）。相反，49期幼虫胸腺中的许多XT-1+细胞是大的（直径8-10 μm）淋巴母细胞样细胞。在第46阶段（受精后5天）胸腺切除术耗尽XT-1+细胞在幼虫和成人淋巴器官的背景水平。这些结果表明，XT-1+细胞在小淋巴细胞出现之前就从胸腺中的淋巴前体细胞分化出来，并迁移到外周淋巴器官中。XT-1单抗识别的细胞表面决定簇可能是爪蟾T淋巴细胞分化的重要标志。
Development of T lymphocytes inXenopus laeviswas studied using a mouse monoclonal antibody (mAb), XT-1, that was produced against surface determinants on thymocytes of J strain frog. Ontogenic studies, employing immunofluorescence, showed that cells positive for the determinant recognized by XT-1 mAb (XT-1+cells) were first detected in the thymus of J strainXenopusby Nieuwkoop and Faber stage 48 (7 days postfertilization) and then in the spleen, liver and kidney by stage 52 (20 days postfertilization). Percentages of XT-1+cells in the thymus increased rapidly by stage 49 (10 days postfertilization) and reached adult levels by stage 52, and those in the spleen, liver, and kidney reached adult levels by stage 56 (40 days postfertilization). Electron microscopic immunohistochemistry revealed that most XT-1+cells in thymuses from stage 56 larvae were typical small lymphocytes (4–7 μm in diameter). In contrast, many XT-1+cells in larval thymuses at stage 49 are large (8–10 μm in diameter) lymphoblastoid cells. Thymectomy at stage 46 (5 days postfertilization) depleted XT-1+cells in larval and adult lymphoid organs to background levels. These results suggest that the XT-1+cells are differentiated from the lymphoid precursor cells in the thymus before the appearance of small lymphocytes and migrate into peripheral lymphoid organs. The cell surface determinant recognized by the XT-1 mAb may provide an important marker for the differentiation of T lymphocytes inXenopus.
在青蛙个体发育过程中，脾细胞对 PHA 和 Con A 的反应性先于胸腺细胞。
影响因子：
6.4
作者：
Rollins-Smith,LA;Parsons,SC;Cohen,N
通讯作者：
Cohen,N
二倍体/三倍体嵌合非洲爪蟾淋巴细胞胚胎起源的流式细胞术分析。
影响因子：
2.7
作者：
Flajnik,MF;Horan,PK;Cohen,N
通讯作者：
Cohen,N