Vibration accelerates orthodontic tooth movement by inducing osteoclastogenesis via transforming growth factor-β signalling in osteocytes
Vibration accelerates orthodontic tooth movement by inducing osteoclastogenesis via transforming growth factor-β signalling in osteocytes
复制标题
振动通过骨细胞中转化生长因子-β 信号传导诱导破骨细胞生成，加速正畸牙齿移动
DOI：
10.1093/ejo/cjac036
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发表时间：
2022
影响因子：
2.6
通讯作者：
Takano-Yamamoto Teruko
中科院分区：
文献类型：
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作者：
Sasaki Kiyo;Takeshita Nobuo;Fukunaga Tomohiro;Seiryu Masahiro;Sakamoto Mayuri;Oyanagi Toshihito;Maeda Toshihiro;Takano-Yamamoto Teruko
背景我们以前在一个实验性的大鼠牙齿移动模型中发现了加速牙齿移动和诱导骨吸收的补充振动条件。然而，补充振动诱导正畸牙齿移动的分子生物学机制尚不完全清楚。转化生长因子-β通过诱导核因子-kappaB受体激活物配体的表达上调破骨细胞的生成，因此转化生长因子-β被认为是诱导骨吸收的重要细胞因子。本研究旨在探讨转化生长因子-β在辅助振动加速正畸牙移动中的作用。分别在第0、7、14、21天对上颌第一磨牙施加辅助振动(3g，70 Hz)3min。将转化生长因子-β受体阻滞剂SB431542注入上颌第一磨牙粘膜下、腭部和颊下，隔日1次。采用RAW264.7细胞和MLO-Y4细胞共培养作为体外破骨细胞形成模型。结果SB431542抑制了实验大鼠牙齿移动模型中补充振动促进牙齿移动和破骨细胞数量的增加。免疫组织化学分析显示，在实验的牙齿移动过程中，补充振动增加了压缩侧牙槽骨中转化生长因子-β-1阳性的骨细胞的数量。结论补充振动通过促进骨细胞产生转化生长因子-β-1而促进正畸牙移动，进而促进破骨细胞的形成。
BackgroundWe previously found the conditions of supplementary vibration that accelerated tooth movement and induced bone resorption in an experimental rat tooth movement model. However, the molecular biological mechanisms underlying supplementary vibration-induced orthodontic tooth movement are not fully understood. Transforming growth factor (TGF)-β upregulates osteoclastogenesis via induction of the receptor activator of nuclear factor kappa B ligand expression, thus TGF-β is considered an essential cytokine to induce bone resorption.ObjectivesThe aim of this study is to examine the role of TGF-β during the acceleration of orthodontic tooth movement by supplementary vibration.Materials and methodsIn experimental tooth movement, 15 g of orthodontic force was loaded onto the maxillary right first molar for 28 days. Supplementary vibration (3 g, 70 Hz) was applied to the maxillary first molar for 3 min on days 0, 7, 14, and 21. TGF-β receptor inhibitor SB431542 was injected into the submucosal palatal and buccal areas of the maxillary first molars once every other day. The co-culture of RAW264.7 cells and MLO-Y4 cells was used as anin vitroosteoclastogenesis model.ResultsSB431542 suppressed the acceleration of tooth movement and the increase in the number of osteoclasts by supplementary vibration in our experimental rat tooth movement model. Immunohistochemical analysis showed supplementary vibration increased the number of TGF-β1-positive osteocytes in the alveolar bone on the compression side during the experimental tooth movement. Moreover, vibration-upregulated TGF-β1 in MLO-Y4 cells induced osteoclastogenesis.ConclusionsOrthodontic tooth movement was accelerated by supplementary vibration through the promotion of the production of TGF-β1 in osteocytes and subsequent osteoclastogenesis.