A diphenol oxidase gene is part of a cluster of genes involved in catecholamine metabolism and sclerotization in Drosophila. II. Molecular localization of the Dox-A2 coding region.
A diphenol oxidase gene is part of a cluster of genes involved in catecholamine metabolism and sclerotization in Drosophila. II. Molecular localization of the Dox-A2 coding region.
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二酚氧化酶基因是果蝇儿茶酚胺代谢和硬化相关基因簇的一部分。
DOI：
10.1093/genetics/112.4.843
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发表时间：
1986
期刊：
影响因子：
3.3
通讯作者：
Wright,TR
中科院分区：
文献类型：
--
作者：
Pentz,ES;Wright,TR
dox -A2（2-53.9）位点的突变改变了双酚氧化酶的A2组分，一种在角质层形成中起重要作用的酶。该位点位于多巴脱羧酶Df(2L) tw130区域，该区域包含至少14个基因，参与果蝇儿茶酚胺代谢和角质层的形成、硬化和黑化。该区域被缺陷细分，克隆DNA的断点定位显示，在近ddc的23 kb中有6个基因密集的亚簇。5个致死性远端基因座包括第二个这样的亚簇。df (2L)hk18和df (2L) od15缺陷的近端断点定义了一个14.3- 16.8 kb的区域，包含了dox - a2和1 (2)37Bb, df (2L) od15和df (2L) tw203缺陷的近端断点定义了一个9.3- 12.1 kb的区域，包含了1 (2)37Ba, 1 (2) 37b和1 (2)37Be。-Southern blots显示两个dox - a2突变是小的缺失（0.1和1.1 kb）。dox - a2locus mRNA为1.7 kb。cDNA克隆表明，3'端位于着丝粒近端，编码区包含至少一个小内含子。dox - a2locus位于df (2L) od15断点的3.4到4.4 kb内，将四个重要的位点放置在最大15.5 kb内。讨论了dox - a2在影响角质层形成的一组基因中的位置。
Mutations at theDox-A2(2-53.9) locus alter the A2 component of diphenol oxidase, an enzyme having an important role in cuticle formation. This locus is in the dopa decarboxylase,Df(2L)TW130region, which contains a cluster of at least 14 genes involved in catecholamine metabolism and the formation, sclerotization and melanization of cuticle in Drosophila. The region is subdivided by deficiencies, and localization of breakpoints in cloned DNA reveals a dense subcluster of six genes in the 23 kb proximal toDdc. Five lethal loci distal toDdccomprise a second such subcluster. The proximal breakpoints of deficienciesDf(2L)hk18andDf(2L)OD15define a 14.3- to 16.8-kb region containingDox-A2andl(2)37Bb, and those ofDf(2L)OD15andDf(2L)TW203define a 9.3- to 12.1-kb region containingl(2)37Ba, l(2)37Bcandl(2)37Be.—Southern blots show two of theDox-A2mutations are small deletions (0.1 and 1.1 kb). TheDox-A2locus mRNA is 1.7 kb. cDNA clones indicate that the 3' end is centromere proximal and that the coding region contains at least one small intron. TheDox-A2locus is within 3.4 to 4.4 kb of theDf(2L)OD15breakpoint, placing four of the vital loci within a maximum of 15.5 kb. The location ofDox-A2in a cluster of genes affecting cuticle formation is discussed.