Diversity of Hepatozoon species in naturally infected dogs in the southern United States
Diversity of Hepatozoon species in naturally infected dogs in the southern United States
复制标题
DOI：
10.1016/j.vetpar.2008.03.027
复制
发表时间：
2008-07-04
影响因子：
2.6
通讯作者：
Little, Susan E.
中科院分区：
文献类型：
--
作者：
Allen, Kelly E.;Li, Yihang;Little, Susan E.
美洲肝虫是一种原生动物，可导致美国南部的美洲犬肝虫病（ACH）;肝虫卡是非洲、亚洲、欧洲和南美洲犬肝虫病的病原体，此前尚未在美国的狗中明确发现。目的研究肝体动物的多样性。在来自俄克拉荷马州的家犬中，通过巢式PCR评价了从居住在该州流行区的犬采集的血液样本、向兽医报告的具有ACH症状的临床病例以及在当地收容所饲养的犬，所述巢式PCR设计用于扩增血液复形虫（包括肝动物属）的18 S rRNA基因的可变区。从ACH流行地区的一只犬、临床患病犬和一只收容所犬中回收的肝体虫序列与H.美国。然而，另外两只收容所的狗有感染H的证据。犬科动物或密切相关的生物体。随后对来自奥本大学分子诊断实验室的实时PCR结果进行了审查，结果显示，来自美国各地的狗提交的大多数样本对肝虫属检测呈阳性。有H。美国。然而，也发现一些提交的材料包含H的DNA序列。犬。混合H. americanum和H.此外，亦发现犬类感染个案。我们的数据表明H. americanum，H. canis和H.在美国南部存在犬样微生物，并可能导致犬疾病。（C）2008 Elsevier B. V.保留所有权利。
Hepatozoon americanum is a protozoan that causes American canine hepatozoonosis (ACH) in the southern United States; Hepatozoon cards, the causative agent of canine hepatozoonosis in Africa, Asia, Europe, and South America, has not previously been definitively identified in dogs in the United States. To characterize the diversity of Hepatozoon spp. in domestic dogs from Oklahoma, blood samples collected from dogs residing in an endemic area of the state, clinical cases presented to veterinarians with symptoms of ACH, and dogs housed at a local shelter were evaluated by a nested PCR designed to amplify a variable region of the 18S rRNA gene of blood ampicomplexa, including Hepatozoon spp. Hepatozoon sequences recovered from a dog from an area where ACH is endemic, from clinically ill dogs, and from one shelter dog most closely resembled H. americanum. However, two other shelter dogs had evidence of infection with H. canis or a closely related organism. A subsequent review of real-time PCR results from the Molecular Diagnostics Laboratory at Auburn University revealed that the majority of samples submitted from dogs from across the United States which tested positive for Hepatozoon spp. had H. americanum. However, some submissions were also found which contained DNA sequence of H. canis. Mixed H. americanum and H. canis-like infections also were detected. Our data suggest that H. americanum, H. canis, as well as H. canis-like organisms are present and may cause disease in dogs in the southern U.S. (C) 2008 Elsevier B.V. All rights reserved.