Comparative efficacy of seven selective media for isolating Campylobacter jejuni
Comparative efficacy of seven selective media for isolating Campylobacter jejuni
复制标题
七种选择性培养基分离空肠弯曲菌的效果比较
DOI：
10.1128/jcm.24.3.451-452.1986
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发表时间：
1986
影响因子：
9.4
通讯作者：
A. C. Velasco
中科院分区：
文献类型：
--
作者：
F. Merino;A. Agulla;P. Villasante;A. Díaz;J. V. Saz;A. C. Velasco
将263例患者的腹泻粪便接种到7种选择性培养基上：Butzler选择性培养基、Blaser培养基、Skirrow血琼脂、普雷斯顿弯曲杆菌选择性培养基、普雷斯顿弯曲杆菌无血培养基、Butzler病毒粒子培养基和改良普雷斯顿培养基（含阿替霉素B [2 mg/L]）。从研究的所有培养基中分离出相似数量的空肠弯曲菌菌株;然而，竞争性粪便植物群（FF）的存在使得可疑菌落的检测变得困难。含头孢哌酮的普雷斯顿弯曲杆菌无血培养基产生的C.空肠分离物，污染FF仅在9%的显示C.空肠生长;所有其他培养基允许其他FF的大量生长，无论C.是否分离空肠。
Diarrheal stools from 263 patients were inoculated on seven selective media: Butzler selective medium, Blaser medium, Skirrow blood agar, Preston campylobacter selective medium, Preston campylobacter blood-free medium, Butzler Virion medium, and modified Preston medium (with amphotericin B [2 mg/liter]). A similar number of Campylobacter jejuni strains were isolated from all the media studied; nevertheless, the presence of competing fecal flora (FF) made the detection of suspect colonies difficult. Preston campylobacter blood-free medium with cefoperazone yielded the greatest number of C. jejuni isolations, and contaminating FF grew in only 9% of the plates showing C. jejuni growth; all the other media allowed the abundant growth of other FF, regardless of whether C. jejuni was isolated from them or not.