Novel rpoA mutation that interferes with the function of OmpR and EnvZ, positive regulators of the ompF and ompC genes that code for outer-membrane proteins in Escherichia coli K12.
Novel rpoA mutation that interferes with the function of OmpR and EnvZ, positive regulators of the ompF and ompC genes that code for outer-membrane proteins in Escherichia coli K12.
复制标题
新型 rpoA 突变会干扰 OmpR 和 EnvZ 的功能，OmpR 和 EnvZ 是编码大肠杆菌 K12 外膜蛋白的 ompF 和 ompC 基因的正调节因子。
DOI：
10.1016/0022-2836(87)90489-x
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发表时间：
1987
影响因子：
5.6
通讯作者：
Shoji Mizushima
中科院分区：
文献类型：
--
作者：
S. Matsuyama;Shoji Mizushima
编码大肠杆菌主要外膜蛋白的ompF和ompC基因的表达受ompRandenv Z基因产物的正调控。最近，我们分离到ompR77突变，其抑制envZ11突变。在这项工作中，一个新的突变，干扰抑制envZ11突变ompR77被分离。该突变位于编码依赖DNA的RNA聚合酶α亚基的therpoA基因。这些结果表明，正调控因子和RNA聚合酶之间的相互作用参与了ompF和ompC基因转录的启动。此外，结果表明，在转录过程中，RNA聚合酶沿沿着DNA链迁移，α亚基朝后，ββ′亚基朝前。
The expression of theompFandompCgenes that code for major outer-membrane proteins ofEscherichia coliis positively regulated by the products of theompRandenvZgenes. Recently, we isolated theompR77mutation, which suppresses theenvZ11mutation. In this work, a novel mutation that interferes with the suppression of theenvZ11mutation byompR77was isolated. The mutation was located in therpoAgene that codes for the α subunit of DNA-dependent RNA polymerase. These results suggest that an interaction between the positive regulators and RNA polymerase is involved in the initiation of transcription of theompFandompCgenes. In addition, the results suggest that during transcription the RNA polymerase migrates along DNA strands with the α subunit facing backward and the ββ′ subunits facing forward.