Expanded mitochondrial control region sequences increase resolution of stock structure among North Atlantic loggerhead turtle rookeries
Expanded mitochondrial control region sequences increase resolution of stock structure among North Atlantic loggerhead turtle rookeries
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扩展的线粒体控制区序列提高了北大西洋红海龟群落种群结构的分辨率
DOI：
10.3354/meps09980
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
D. Warnell
中科院分区：
文献类型：
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作者：
B. Shamblin;A. Bolten;K. Bjorndal;P. Dutton;J. Nielsen;F. Abreu;K. J. Reich;B. Witherington;D. Bagley;L. Ehrhart;A. Tucker;D. Addison;A. Arenas;Chris Johnson;R. Carthy;Margaret M. Lamont;Mark G. Dodd;M. Gaines;E. LaCasella;C. Nairn;D. Warnell
美国东南部是大西洋上最大的斑海龟聚居地。在墨西哥加勒比海沿岸、古巴和巴哈马群岛也出现了显著的区域筑巢现象。以前基于线粒体控制区380bp片段对北大西洋斑嘴龟栖息地的研究支持识别出除了东大西洋的佛得角外，西北大西洋还有8个种群统计上独立的筑巢种群(管理单位)。最近对扩展的线粒体控制区序列的分析表明，西部和东部大西洋斑龟栖息地之间存在额外的遗传多样性和增加的种群结构。我们对来自美国东南部、巴哈马的Cay Sal Bank和墨西哥的Quintana Roo的2427个样本的817个核苷酸的线粒体DNA片段进行了测序。FST成对比较、种群分化的成对精确检验和分子方差分析支持先前提出的管理单元命名，并另外表明，佛罗里达东南部和西南部的雏鸟应被确认为不同的管理单元。因此，根据(1)弗吉尼亚州至佛罗里达州东北部、(2)佛罗里达州中部东部、(3)佛罗里达州东南部、(4)佛罗里达州托尔图加斯干地、(5)巴哈马珊瑚礁、(6)古巴西南部、(7)墨西哥昆塔纳鲁奥、(8)佛罗里达州西南部、(9)佛罗里达州中西部和(10)佛罗里达州西北部海滩，西北部大西洋斑龟栖息地可细分为10个管理单元。我们证实，许多西北大西洋管理单位和佛得角菜鸟之间的库存结构分辨率有所提高，并扩大了控制区单倍型。
The southeastern USA hosts the largest nesting concentration of loggerhead turtles Caretta caretta in the Atlantic. Regionally significant nesting also occurs along the Caribbean coast of Mexico, in Cuba, and in the Bahamas. Previous studies of North Atlantic loggerhead turtle rookeries based on a 380 bp fragment of the mitochondrial control region supported recognition of 8 demographically independent nesting populations (management units) in the Northwest Atlantic in addition to Cape Verde in the eastern Atlantic. Recent analysis of expanded mitochon- drial control region sequences revealed additional genetic diversity and increased population structure between western and eastern Atlantic loggerhead turtle rookeries. We se quenced an 817 bp mitochondrial DNA fragment in 2427 samples from nesting beaches in the southeastern USA, Cay Sal Bank, Bahamas, and Quintana Roo, Mexico. Pairwise FST comparisons, pairwise exact tests of population differentiation, and analysis of molecular variance support previously proposed management unit designations and additionally indicate that southeastern and south- western Florida rookeries should be recognized as distinct management units. Therefore, North- west Atlantic loggerhead turtle rookeries can be subdivided into 10 management units, corre- sponding to the beaches from (1) Virginia through northeastern Florida, (2) central eastern Florida, (3) southeastern Florida, (4) Dry Tortugas, Florida, (5) Cay Sal, Bahamas, (6) southwestern Cuba, (7) Quintana Roo, Mexico, (8) southwestern Florida, (9) central western Florida, and (10) northwestern Florida. We confirmed increased resolution of stock structure between many North- west Atlantic management units and the Cape Verde rookery with the expanded control region haplotypes.