Cyclin-dependent kinase 4 is a novel target in micoRNA-195-mediated cell cycle arrest in bladder cancer cells
Cyclin-dependent kinase 4 is a novel target in micoRNA-195-mediated cell cycle arrest in bladder cancer cells
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细胞周期蛋白依赖性激酶 4 是 micoRNA-195 介导的膀胱癌细胞细胞周期停滞的新靶点
DOI：
10.1016/j.febslet.2012.01.027
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发表时间：
2012-02-17
期刊：
影响因子：
3.5
通讯作者：
Xie, Liping
中科院分区：
文献类型：
--
作者：
Lin, Yiwei;Wu, Jian;Xie, Liping
miRNAs是一类能够负调控基因表达的小分子非编码RNA。在这里，我们发现miR-195在人膀胱癌组织中相对于正常邻近组织下调。为了更好地表征miR-195在膀胱癌中的作用，我们通过用化学合成的miR-195模拟物转染膀胱癌细胞系T24进行了功能分析。我们确定了CDK 4，一种早期G1细胞周期调节因子，作为miR-195的新靶点。选择性过表达miR-195可诱导T24细胞发生G1期阻滞，进而抑制T24细胞生长。这些发现表明miR-195可能是膀胱癌的潜在肿瘤抑制因子。(C)2012年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
miRNAs are a class of small-noncoding RNAs capable of negatively regulating gene expression. Here, we found that miR-195 is down-regulated in human bladder cancer tissue versus normal adjacent tissue. To better characterize the role of miR-195 in bladder cancer, we conducted gain of function analysis by transfecting bladder cancer cell line T24 with chemically synthesized miR-195 mimic. We identified CDK4, an early G1 cell cycle regulator, as a novel target of miR-195. Selective overexpression of miR-195 could induce G1-phase arrest in T24 cells, and subsequently inhibit T24 cell growth. These findings indicate that miR-195 could be a potential tumor suppressor in bladder cancer. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.