Cardiac progenitor cells in brown adipose tissue repaired damaged myocardium
Cardiac progenitor cells in brown adipose tissue repaired damaged myocardium
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DOI：
10.1016/j.bbrc.2006.01.181
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发表时间：
2006-04-07
影响因子：
3.1
通讯作者：
Takakura, N
中科院分区：
文献类型：
--
作者：
Yamada, Y;Wang, XD;Takakura, N
心肌细胞(CM)的再生在成人生活中是有限的，不足以预防心肌梗死。因此，确定一个有用的CM祖细胞来源对于可能用于再生治疗是非常有意义的。骨髓中的间充质干细胞、胚胎干细胞和骨骼肌成肌细胞是CM补充的已知来源；然而，在临床应用中存在一些关键问题。在本研究中，我们成功地鉴定了棕色脂肪组织(BAT)中的CM祖细胞。此外，我们还发现，CB29阳性群体中存在的BAT的CM祖细胞可以高效地分化为CM。为了证实CD29(+)BAT来源细胞(BATDC)的体内效应，我们将这些细胞移植到大鼠急性心肌梗死模型的梗死区。结果表明，与CD29(+)白色脂肪组织来源的细胞或生理盐水相比，CD29(+)的BATDC移植后，心肌梗死面积缩小，左心功能改善。这些发现表明，BATDC是CM再生新策略的有用来源之一。(C)2006 Elsevier Inc.保留所有权利。
Cardiomyocyte (CM) regeneration is limited in adult life and is not sufficient to prevent myocardial infarction. Hence, the identification of a useful source of CM progenitors is of great interest for possible use in regenerative therapy. Mesenchymal stem cells in bone marrow, embryonic stem cells, and skeletal myoblasts are known sources of CM repletion; however, there are a number of critical problems for clinical application. In this study, we succeeded to identify CM progenitor cells in brown adipose tissue (BAT). Moreover, we showed that CM progenitor cells in BAT that existed in CB29-positive population could differentiate into CM with high efficiency. To confirm the in vivo effect of CD29(+)BAT-derived cells (BATDCs), we transplanted these cells into infarct border zone of an acute myocardial infarction model in rat. Results clearly indicated that implantation of CD29(+) BATDCs led to the reduction of the infarction area and improvement of left ventricular function by replacing newly developed CMs in comparison with that by CD29(+) white adipose tissue-derived cells or control saline. These findings suggest that BATDCs are one of the useful sources for a new strategy in CM regeneration. (c) 2006 Elsevier Inc. All rights reserved.