SUPPRESSION OF LYMPHOCYTE MITOGENESIS BY TAMOXIFEN
SUPPRESSION OF LYMPHOCYTE MITOGENESIS BY TAMOXIFEN
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DOI：
10.1016/0162-3109(89)90030-1
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发表时间：
1989-07-01
期刊：
影响因子：
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通讯作者：
BERCZI, I
中科院分区：
文献类型：
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作者：
BARAL, E;KWOK, S;BERCZI, I
他莫昔芬(0.1-1 .mu。M浓度)显著抑制大鼠脾细胞对植物血凝素、豆豆蛋白A、商陆丝裂原和脂质A的反应，且呈剂量依赖性。为了达到这种抑制作用，在有丝分裂刺激前将淋巴细胞暴露于他莫昔芬4小时就足够了。在相同条件下，雌二醇对淋巴细胞有丝分裂的影响并不一致，也没有改变他莫昔芬的抑制作用，即使淋巴细胞先用雌二醇后再用他莫昔芬。提示他莫昔芬对这些体外淋巴细胞反应的抑制作用不是由雌激素受体介导的。
Tamoxifen (at 0.1-1 .mu.M concentrations) inhibited significantly the response of rat spleen cells to phytohemagglutinin, concanavalin A, pokeweed mitogen and lipid A in a dose-dependent manner. To achieve this inhibition, it was sufficient to expose the lymphocytes for 4 h to tamoxifen prior to mitogenic stimulation. Estradiol did not have a consistent effect on lymphocyte mitogenesis under the same conditions and did not modify the suppressive effect of tamoxifen, even when the lymphocytes were treated first with estradiol followed by tamoxifen. It is suggested that the inhibitory effect of tamoxifen on these in vitro lymphocyte reactions is not mediated by the estrogen receptor.