Differential expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in ulcerative colitis and Crohn's disease
Differential expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in ulcerative colitis and Crohn's disease
复制标题
DOI：
10.1046/j.1440-1827.2002.01365.x
复制
发表时间：
2002-05-01
影响因子：
2.2
通讯作者：
Nagura, H
中科院分区：
文献类型：
--
作者：
Arihiro, S;Ohtani, H;Nagura, H
粘蛋白地址素细胞粘附分子-1（MAdCAM-1）选择性表达于肠粘膜内皮细胞和肠相关淋巴组织（GALT）。MAdCAM-1与淋巴细胞上其配体整合素α（4）β（7）的结合与肠道相关淋巴细胞归巢至正常胃肠道和炎症部位有关。本研究旨在阐明克罗恩病（CrD）和溃疡性结肠炎（UC）之间的差异，从MAdCAM-1的表达模式。在手术切除时，从40例CrD患者和24例UC患者中采集样本。使用冷冻切片，对MAdCAM-1、E-选择素和CD 34进行免疫组织化学。UC和CrD的炎症粘膜中MAdCAM-1（+）微静脉丰富。与此相反，在溃疡基底部的炎症区域中，在UC和CrD之间注意到明显的差异，即MAdCAM-1（+）微静脉在CrD中比在UC中更丰富（P < 0.001），而E-选择素在两种疾病的这些微静脉中表达相等。此外，CrD的特征在于在肠组织的深层中出现MAdCAM-1（+）小静脉，主要是在淋巴聚集体中。我们的数据表明MAdCAM-1在CrD中更广泛的表达，这不仅可能导致粘膜炎症，而且可能导致CrD中的透壁炎症。
Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is selectively expressed in the endothelial cells of intestinal mucosa and gut-associated lymphoid tissue (GALT). Engagement of MAdCAM-1 to its ligand, integrin alpha(4)beta(7) , on lymphocytes is associated with the homing of gut-associated lymphocytes to normal gastrointestinal tract and inflammation sites. The present study was designed to elucidate differences between Crohn's disease (CrD) and ulcerative colitis (UC) from the expression patterns of MAdCAM-1. Samples were taken from 40 patients with CrD and 24 patients with UC at surgical resection. Using frozen sections, immunohistochemistry was performed for MAdCAM-1, E-selectin and CD34. MAdCAM-1(+) venules were abundant in inflamed mucosa in both UC and CrD. In contrast, clear differences were noted between UC and CrD in the inflammatory area in the ulcer base, that is, MAdCAM-1(+) venules were more abundant in CrD than in UC (P < 0.001), while E-selectin was expressed equally in these venules in both diseases. Furthermore, CrD was characterized by the occurrence of MAdCAM-1(+) venules in deeper layers of the intestinal tissue, mainly in lymphoid aggregates. Our data indicated more extensive expression of MAdCAM-1 in CrD, which could contribute not only to mucosal inflammation, but also to transmural inflammation in CrD.