THE FISSION YEAST CUT1+ GENE REGULATES SPINDLE POLE BODY DUPLICATION AND HAS HOMOLOGY TO THE BUDDING YEAST ESP1 GENE
THE FISSION YEAST CUT1+ GENE REGULATES SPINDLE POLE BODY DUPLICATION AND HAS HOMOLOGY TO THE BUDDING YEAST ESP1 GENE
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DOI：
10.1016/0092-8674(90)90266-h
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发表时间：
1990-09-07
期刊：
影响因子：
64.5
通讯作者：
YANAGIDA, M
中科院分区：
文献类型：
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作者：
UZAWA, S;SAMEJIMA, I;YANAGIDA, M
裂变酵母cut 1-、cut 2+和cut 10+基因的突变使正常协调的有丝分裂事件解偶联，使有丝分裂解除调节，而不是停滞。DNA合成继续进行，使多倍体细胞核与几个纺锤体。在cut 1突变细胞中产生多个异常的纺锤体极体（SPB）。通过转化克隆cut 1+和cut 2+基因。高基因剂量的cut 1+也补充了cut 2和cut 10突变体。然而，cut 2+基因仅补充cut 2。210 kd cut 1+基因产物含有推定的ATP结合和螺旋卷曲区域，随后是与酿酒酵母基因ESP 1同源的COOH末端结构域。ESP 1基因的突变也会导致许多SPB。抗cut 1抗体显示cut 1产物是不溶性核组分的罕见组分。它可能在染色体分离与其他细胞周期事件的耦合中发挥关键作用，并且可能是SPB和/或着丝粒的组分、调节剂或马达。
Mutations in the fission yeast cut1-, cut2+, and cut10+ genes uncouple normally coordinated mitotic events and deregulate, rather than arrest, mitosis. DNA synthesis continues, making polyploid nuclei with several spindles. Multiple, aberrant spindle pole bodies (SPBs) are produced in cut1 mutant cells. The cut1+ and cut2+ genes are cloned by transformation. High gene dosage of cut1+ also complements cut2 and cut10 mutants. The cut2+ gene, however, complements only cut2. The 210 kd cut1+ gene product contains putative ATP binding and helical coil regions followed by a COOH-terminal domain homologous to the Saccharomyces cerevisiae gene ESP1. Mutations in the ESP1 gene also result in many SPBs. The cut1- product is shown by anti-cut1 antibody to be a rare component of the insoluble nuclear fraction. It may play a key role in coupling chromosome disjunction with other cell cycle events and is potentially a component, regulator, or motor for the SPB and/or kinetochores.