Two methods to analyze LRRK2 functions under lysosomal stress: the measurements of cathepsin release and lysosomal enlargement.
Two methods to analyze LRRK2 functions under lysosomal stress: the measurements of cathepsin release and lysosomal enlargement.
复制标题
分析溶酶体应激下 LRRK2 功能的两种方法：组织蛋白酶释放和溶酶体增大的测量。
DOI：
10.1007/978-1-0716-1495-2_7
复制
发表时间：
2021
影响因子：
--
通讯作者：
Kuwahara T.
中科院分区：
文献类型：
--
作者：
Sakurai M.;Kuwahara T.
富含亮氨酸重复序列激酶2（LRRK2）是常染色体显性帕金森病的致病基因产物，并已被证明在溶酶体调节中发挥作用。我们先前已经表明，内源性LRRK2招募其底物Rab8a和Rab10到超载的溶酶体上，这取决于它们的磷酸化，其功能是抑制溶酶体扩大以及促进溶酶体组织蛋白酶的胞吐释放。在本章中，我们介绍了两种方法来分析LRRK2暴露于RAW264.7细胞中溶酶体过载应激后的细胞功能。
Leucine-rich repeat kinase 2 (LRRK2) is a causative gene product of autosomal-dominant Parkinson’s disease and has been shown to play a role in lysosomal regulation. We have previously shown that endogenous LRRK2 recruited its substrates Rab8a and Rab10 onto overloaded lysosomes depending on their phosphorylation, which functioned in the suppression of lysosomal enlargement as well as the promotion of the exocytic release of lysosomal cathepsins. In this chapter, we introduce two methods to analyze cellular functions of LRRK2 upon exposure to lysosomal overload stress in RAW264.7 cells.