Influences of hucMSC-exosomes on VEGF and BMP-2 expression in SNFH rats
Influences of hucMSC-exosomes on VEGF and BMP-2 expression in SNFH rats
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DOI：
10.26355/eurrev_201904_17573
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发表时间：
2019-04-01
影响因子：
3.3
通讯作者：
Zhang, G. -C.
中科院分区：
文献类型：
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作者：
Li, R.;Chen, C.;Zhang, G. -C.
目的：探讨人脐带间充质干细胞来源的外泌体对人脐带间充质干细胞增殖的影响。（hucMSC-exosome）对大鼠激素诱导的股骨头坏死（SNFH）以及血管内皮生长因子（VEGF）和骨形态发生蛋白-2（BMP-2）表达的影响。采用随机数字表将20只雄性Sprague-Dawley大鼠随机分为SNFH组和SNFH + hucMSC-exosome组。每周2次腹腔注射醋酸泼尼松龙（24.5mg/kg）建立SNFH大鼠模型，SNFH + hucMSC-exosome组在此基础上骨髓腔内注射一定剂量的hucMSC-exosome。3周后，通过苏木精-伊红（H&E）染色和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）染色检测hucMSC-exosome对SNFH大鼠股骨头病理变化和细胞凋亡的影响。采用免疫组化法检测两组骨组织中分化抗原31（CD 31）、VEGF、BMP-2的表达，采用逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法检测两组坏死骨组织中VEGF、BMP-2的mRNA和蛋白表达水平。H&E染色结果显示，SNFH + hucMSC-exosome组纤维骨痂形成较好，新生骨小梁结构较明显，真空裂隙数量减少，脂肪细胞增大较少。TUNEL染色结果显示SNFH + hucMSC-exosome组股骨头组织中凋亡细胞数量较少（p
OBJECTIVE: To investigate the influences of human umbilical cord mesenchymal stem cell-derived exosomes (hucMSC-exosome) on steroid-induced necrosis of the femoral head (SNFH) and the expressions of vascular endothelial growth factor (VEGF) and bone morphogenetic protein-2 (BMP-2) in rats.PATIENTS AND METHODS: A total of 20 male Sprague-Dawley rats were randomly divided into SNFH group and SNFH + hucMSC-exosome group using a random number table. Prednisolone acetate (24.5 mg/kg) was injected twice a week to establish the rat model of SNFH, and hucMSC-exosome in a certain dose was additionally injected into the marrow cavity in SNFH + hucMSC-exosome group. After 3 weeks, the influences of hucMSC-exosome on the pathological changes and apoptosis of the femoral head in SNFH rats were detected via hematoxylin-eosin (H&E) staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. In addition, the expressions of cluster of differentiation 31 (CD31), VEGF, and BMP-2 in bone tissues in both groups were detected via immunohistochemical staining, and the messenger ribonucleic acid (mRNA) and protein expression levels of VEGF and BMP-2 in necrotic bone tissues in both groups were detected via Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Western blotting.RESULTS: The results of H&E staining revealed that the fibrous callus formation was good, the new trabecular structure was more obvious, the number of vacuum cleft declined, and there were fewer enlarged adipocytes in SNFH + hucMSC-exosome group compared with SNFH group. The results of TUNEL staining showed that the number of apoptotic cells in femoral head tissues was smaller in SNFH + hucMSC-exosome group (p