Physicochemical functionality of chimeric isomaltomegalosaccharides with α-(1?→?4)-glucosidic segments of various lengths
Physicochemical functionality of chimeric isomaltomegalosaccharides with α-(1?→?4)-glucosidic segments of various lengths
复制标题
不同长度α-(1→→4)-糖苷片段嵌合异麦芽糖的理化功能
DOI：
10.1016/j.carbpol.2022.119562
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发表时间：
2022
影响因子：
11.2
通讯作者：
Kimura Atsuo
中科院分区：
文献类型：
--
作者：
Lang Weeranuch;Kumagai Yuya;Habu Shinji;Sadahiro Juri;Tagami Takayoshi;Okuyama Masayuki;Kitamura Shinichi;Sakairi Nobuo;Kimura Atsuo
异麦芽糖（IMS）是一种长链嵌合葡萄糖，由α-（1 → 6）-和α-（1 → 4）-连接的片段分别在非还原和还原端组成;这些片段的亲水性和疏水性预期导致双功能性。我们酶促合成IMS，其平均聚合度（DP）为15.8、19.3和23.5，其中α-（1 → 4）-片段的DP分别为3、6和9。IMS由于α-（1 → 6）-链段而表现出比麦芽糊精高得多的水溶性，并且具有与短葡聚糖相同的耐热降解性。IMS与荧光探针2-对甲苯胺基萘-6-磺酸盐的相互作用表明，IMS比麦芽糊精具有更强的疏水性，其疏水性随α-（1 → 4）-链段DP的增加而增加（9 > 6 > 3）。荧光芘估计极性的IMS被发现是类似的甲醇或1-丁醇。双功能IMS提高了槲皮素-3-O-葡萄糖苷和槲皮素的水溶性：增溶难溶性生物活性物质有利于碳水化合物工业。
Isomaltomegalosaccharide (IMS) is a long chimeric glucosaccharide composed of α-(1 → 6)- and α-(1 → 4)-linked segments at nonreducing and reducing ends, respectively; the hydrophilicity and hydrophobicity of these segments are expected to lead to bifunctionality. We enzymatically synthesized IMS with average degrees of polymerization (DPs) of 15.8, 19.3, and 23.5, where α-(1 → 4)-segments had DPs of 3, 6, and 9, respectively. IMS exhibited considerably higher water solubility than maltodextrin because of the α-(1 → 6)-segment and an identical resistance to thermal degradation as short dextran. Interaction of IMS with a fluorescent probe of 2-p-toluidinylnaphthalene-6-sulfonate demonstrated that IMS was more hydrophobic than maltodextrin, where the degree of hydrophobicity increased as DP of α-(1 → 4)-segment increased (9 > 6 > 3). Fluorescent pyrene-estimating polarity of IMS was found to be similar to that of methanol or 1-butanol. The bifunctional IMS enhanced the water solubility of quercetin-3-O-glucoside and quercetin: the solubilization of less-soluble bioactive substances is beneficial in carbohydrate industry.