IMMUNE INTERFERON RELEASE WHEN A CLONED CYTO-TOXIC T-CELL LINE MEETS ITS CORRECT INFLUENZA-INFECTED TARGET-CELL
IMMUNE INTERFERON RELEASE WHEN A CLONED CYTO-TOXIC T-CELL LINE MEETS ITS CORRECT INFLUENZA-INFECTED TARGET-CELL
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DOI：
10.1038/295150a0
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发表时间：
1982-01-01
期刊：
影响因子：
64.8
通讯作者：
ASKONAS, BA
中科院分区：
文献类型：
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作者：
MORRIS, AG;LIN, YL;ASKONAS, BA
有丝分裂原1、特异性抗原2，包括病毒抗原3和同种异体抗原4刺激T细胞产生免疫干扰素(γ)。然而，在T细胞中诱导干扰素-γ的确切情况还没有确定，特别是产生干扰素的细胞类型的确切性质。我们最近报道了一株BALB/c细胞毒性T(TC)细胞系(L4)的筛选和克隆，该细胞系在T细胞生长因子(TCGF)存在下生长，Lyt-2阳性，并能杀死感染任何A型流感病毒的H-2d靶细胞。本研究的目的是检测克隆的细胞毒性T细胞株是否在与适当的靶细胞接触时产生干扰素-γ。结果发现，L4细胞与靶细胞共培养可产生少量干扰素-γ，而L4细胞本身不释放可检测到的干扰素。L4细胞产生干扰素的抗原特异性模式类似于杀灭要求，因为T细胞介导的裂解仅限于感染甲型流感病毒的H-2D靶标，而不是流感病毒B株，并且干扰素的产生不被病毒血凝素的单抗阻止，血凝素在中和病毒的同时不抑制细胞毒性。
T cells stimulated by mitogens1, specific antigens2, including viral antigens3, and alloantigens4have been shown to produce immune Interferon (IFN-γ). However, the exact circumstances of induction of IFN-γin T cells have not yet been established, particularly the precise nature of the cell type producing the Interferon. We have recently described5the selection and cloning of a BALB/c cytotoxic T(Tc)-cell line (L4) which grows in the presence of T-cell growth factor (TCGF), is Lyt-2 positive and kills H-2dtarget cells infected with any type A influenza vims. The aim of the present study was to examine whether this cloned cytotoxic T-cell line produced IFN-γon contact with the appropriate target cell. It was found that small amounts of IFN-γwere produced on co-culture of L4 with target cells, whereas L4 cells on their own released no detectable Interferon. The pattern of antigenic specificity by L4 cells for interferon production resembled the requirements for killing in that Tccell mediated lysis was restricted to H–2dtargets infected with influenza A, but not influenza virus B strains, and interferon production was not prevented by a monoclonal antibody to the virus' haemagglutinin, which does not inhibit cytotoxicity while neutralizing the virus.