Coordination of transcription, processing, and export of highly expressed RNAs by distinct biomolecular condensates.
Coordination of transcription, processing, and export of highly expressed RNAs by distinct biomolecular condensates.
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DOI：
10.1042/etls20190160
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发表时间：
2020-12-11
影响因子：
3.8
通讯作者：
Bungert J
中科院分区：
文献类型：
--
作者：
Ishov AM;Gurumurthy A;Bungert J
在超级增强子控制下的基因以极高的水平表达，并且经常与核斑点相关。最近的数据表明，高浓度的非磷酸化RNA聚合酶II（Pol II）和介体招募到超级增强子创建相分离的缩合物。在这些相分离的液滴内或表面处启动转录，并且与转录启动和延伸相关的Pol II的磷酸化使Pol II与这些结构域解离，导致与富含RNA加工因子的核斑点接合。Pol II从转录起始结构域到RNA加工结构域的转变有效地协调了高表达RNA的转录和加工，然后将其快速输出到细胞质中。
Genes under control of super-enhancers are expressed at extremely high levels and are frequently associated with nuclear speckles. Recent data suggest that the high concentration of unphosphorylated RNA polymerase II (Pol II) and Mediator recruited to super-enhancers create phase-separated condensates. Transcription initiates within or at the surface of these phase-separated droplets and the phosphorylation of Pol II, associated with transcription initiation and elongation, dissociates Pol II from these domains leading to engagement with nuclear speckles, which are enriched with RNA processing factors. The transitioning of Pol II from transcription initiation domains to RNA processing domains effectively co-ordinates transcription and processing of highly expressed RNAs which are then rapidly exported into the cytoplasm.