DETERMINATION OF DNA PERSISTENCE LENGTH BY CRYOELECTRON MICROSCOPY - SEPARATION OF THE STATIC AND DYNAMIC CONTRIBUTIONS TO THE APPARENT PERSISTENCE LENGTH OF DNA
DETERMINATION OF DNA PERSISTENCE LENGTH BY CRYOELECTRON MICROSCOPY - SEPARATION OF THE STATIC AND DYNAMIC CONTRIBUTIONS TO THE APPARENT PERSISTENCE LENGTH OF DNA
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DOI：
10.1006/jmbi.1995.0640
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发表时间：
1995-12-08
影响因子：
5.6
通讯作者：
STASIAK, A
中科院分区：
文献类型：
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作者：
BEDNAR, J;FURRER, P;STASIAK, A
溶液中DNA分子的轴向偏转是由DNA序列的热运动和固有曲率引起的。为了直接测量热运动的贡献，我们构建了固有直链DNA分子，并用低温电子显微镜测量了它们的持续长度。这种固有直链DNA分子悬浮在低温玻璃化溶液薄层中的持续长度约为80 nm。为了测试我们的实验方法，我们测量了具有自然“随机”序列的DNA分子的表观持续长度。约45 nm的结果与公认的天然DNA序列表观持续长度值一致。通过比较天然DNA与固有直链DNA的表观持续长度，可以确定动态和静态对表观持续长度的贡献。(C)1995年学术出版社
Axial deflection of DNA molecules in solution results from thermal motion and intrinsic curvature related to the DNA sequence. In order to measure directly the contribution of thermal motion we constructed intrinsically straight DNA molecules and measured their persistence length by cryo-electron microscopy The persistence length of such intrinsically straight DNA molecules suspended in thin layers of cryo-vitrified solutions is about 80 nm. In order to test our experimental approach, we measured the apparent persistence length of DNA molecules with natural ''random'' sequences. The result of about 45 nm is consistent with the generally accepted value of the apparent persistence length of natural DNA sequences. By comparing the apparent persistence length to intrinsically straight DNA with that of natural DNA, it is possible to determine both the dynamic and the static contributions to the apparent persistence length. (C) 1995 Academic Press Limited