INTRATRACHEAL INSTILLATION METHOD FOR MOUSE LUNGS
INTRATRACHEAL INSTILLATION METHOD FOR MOUSE LUNGS
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DOI：
10.1159/000224748
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发表时间：
1973-01-01
期刊：
影响因子：
3.5
通讯作者：
FURST, A
中科院分区：
文献类型：
--
作者：
HO, W;FURST, A
开发了一种肺内滴注技术，该技术允许将精确体积的颗粒混悬液重复给药至小鼠肺中。以氧化铁吸附苯并（a）芘（BP）为例，验证了该方法的可行性。大多数动物在4周给药后存活。组织学和新鲜组织切片显示两种化学物质广泛渗透到肺实质中;然而，虽然BP从器官中迅速消除，但氧化铁保留超过100天。这些粉尘颗粒在肺的终末细支气管和淋巴组织周围收集的巨噬细胞内观察到。
An intratracheal instillation technique was developed which permits the repeated administrations of precise volumes of particulate suspension into mouse lungs. Benzo(a)pyrene (BP) adsorbed to ferric oxide was used to test the feasibility of the method. The majority of the animals survived 4 weekly treatments. Histological and fresh tissue sections showed extensive penetration of both chemicals into the lung parenchyma; however, while BP was eliminated from the organ rapidly, the ferric oxide was retained for over a 100 days. These dust particles were observed inside macrophages collected around terminal bronchioles and lymphoid tissues of the lungs.