Endothelialization of polyurethanes: Surface silanization and immobilization of REDV peptide
Endothelialization of polyurethanes: Surface silanization and immobilization of REDV peptide
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DOI：
10.1016/j.colsurfb.2016.04.017
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发表时间：
2016-08-01
影响因子：
5.8
通讯作者：
Ciach, Tomasz
中科院分区：
文献类型：
--
作者：
Butruk-Raszeja, Beata A.;Dresler, Magdalena S.;Ciach, Tomasz
本文介绍了精氨酸-谷氨酸-天冬氨酸-缬氨酸(REDV)多肽在聚氨酯表面的化学固定化方法。该多肽以硅烷为间隔物分子共价键合。这项工作的目的是调查拟议的修饰过程并评估其生物学效果，特别是在与血液和内皮细胞接触的情况下。对引入的官能团和多肽分子的润湿性、原子组成和密度等物理化学性质进行了检测。血液实验表明，材料涂层减少了表面附着的血小板和纤维蛋白原分子的数量。与聚氨酯(PU)相比，经REDV修饰的材料(PU-REDV)上没有血液成分沉积；与PU相比，纤维蛋白原在PU-REDV表面的吸附显著减少。培养1、2、3、4和5天后的细胞黏附分析表明，与PU相比，PU-REDV上的细胞被覆面积显著增加。然而，在没有添加REDV的对照表面上也出现了强烈的细胞生长。因此，REDV多肽对人脐静脉内皮细胞黏附的积极作用尚不能得到明确的证实。(C)2016爱思唯尔B.V.保留所有权利。
The paper presents method for chemical immobilization of arginine-glutamic acid-aspartic acid-valine (REDV) peptide on polyurethane surface. The peptide has been covalently bonded using silanes as a spacer molecules. The aim of this work was to investigate the proposed modification process and assess its biological effectiveness, especially in contact with blood and endothelial cells. Physicochemical properties were examined in terms of wettability, atomic composition and density of introduced functional groups and peptide molecules.Experiments with blood showed that material coating reduced number of surface-adhered platelets and fibrinogen molecules. In contrast to polyurethane (PU), there were no blood components deposited on REDV-modified materials (PU-REDV); fibrinogen adsorption on PU-REDV surface has been strongly reduced compared to PU.Analysis of cell adhesion after 1, 2, 3, 4, and 5 days of culture showed a significant increase of the cell coated area on PU-REDV compared to PU. However, an intense cell growth appeared also on the control surface modified without the addition of REDV. Thus, the positive effect of REDV peptide on the adhesion of HUVEC could not be unequivocally confirmed. (C) 2016 Elsevier B.V. All rights reserved.