LF1 regulates the lateral organs polarity development in rice
LF1 regulates the lateral organs polarity development in rice
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LF1调节水稻侧生器官极性发育
DOI：
10.1111/nph.17220
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发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Yunfeng Li
中科院分区：
文献类型：
--
作者：
Ting Zhang;Jing You;Yi Zhang;Wanyue Yao;Wenbo Chen;Qiannan Duan;Wenwen Xiao;Li Ye;Yue Zhou;Xianchun Sang;Yinghua Ling;Guanghua He;Yunfeng Li
近轴-远轴组织的图案化在侧器官的形态中起着至关重要的作用，这是通过指定近轴和远轴组织身份的基因之间的拮抗作用来维持的。同源结构域亮氨酸拉链III类（HD‐ZIP III）家族基因调节近轴身份;然而，关于HD‐ZIP III的物理相互作用或转录调控下游基因的信息很少。在这项研究中，我们鉴定了一个显性水稻突变体，侧小花1（LF 1），它具有侧器官极性缺陷。LF 1编码HD‐ZIP III转录因子，LF 1可直接激活LITTLE ZIPPER家族基因OsZPR 4和HD-ZIP II家族基因OsHOX 1的表达，OsZPR 4和OsHOX 1分别与LF 1相互作用形成异源二聚体，抑制LF 1的转录活性。LF 1通过直接调节OsYUCCA 6的表达来影响吲哚乙酸（IAA）的含量。因此，LF 1在OsZPR 4和OsHOX 1之间形成负反馈环，影响IAA含量，从而调控侧器官极性发育。这些结果揭示了HD‐ZIP III、LITTLE ZIPPER和HD‐ZIP II蛋白之间的相互作用，为侧器官极性发育的分子机制提供了新的见解。
The patterning of adaxial–abaxial tissues plays a vital role in the morphology of lateral organs, which is maintained by antagonism between the genes that specify adaxial and abaxial tissue identity. The homeo‐domain leucine zipper class III (HD‐ZIP III) family genes regulate adaxial identity; however, little information is known about the physical interactions or transcriptionally regulated downstream genes of HD‐ZIP III.In this study, we identified a dominant rice mutant,lateral floret 1(lf1), which has defects in lateral organ polarity.LF1encodes the HD‐ZIP III transcription factor, which expressed in the adaxial area of lateral organs.LF1 can activate directly the expression ofLITTLE ZIPPERfamily geneOsZPR4and HD‐ZIP II family geneOsHOX1, and OsZPR4 and OsHOX1 respectively interact with LF1 to form a heterodimer to repress the transcriptional activity of LF1. LF1 influences indole‐3‐acetic acid (IAA) content by directly regulating the expression ofOsYUCCA6. Therefore, LF1 forms negative feedback loops between OsZPR4 and OsHOX1 to affect IAA content, leading to the regulation of lateral organs polarity development.These results reveal the cross‐talk among HD‐ZIP III, LITTLE ZIPPER, and HD‐ZIP II proteins and provide new insights into the molecular mechanisms underlying the polarity development of lateral organs.