Real-time PCR analysis of HIV-1 replication post-entry events.
Real-time PCR analysis of HIV-1 replication post-entry events.
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DOI：
10.1007/978-1-59745-170-3_5
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Pathak VK
中科院分区：
文献类型：
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作者：
Mbisa JL;Delviks-Frankenberry KA;Thomas JA;Gorelick RJ;Pathak VK
逆转录酶在HIV-1的生命周期中起着重要作用，通过逆转录将单链病毒RNA基因组转化为双链病毒DNA。产生的双链DNA整合到宿主染色体中形成前病毒。一小部分的病毒DNA形成死端环状产物，然而，它可以作为有用的替代标记物用于监测病毒复制。利用实时PCR技术，可以跟踪和定量逆转录过程的不同阶段、前病毒和非整合的死端逆转录产物。
The reverse transcriptase enzyme plays an essential role in the HIV-1 life cycle by converting a singlestranded viral RNA genome into a double-stranded viral DNA through a complex process known as reverse transcription. The resulting double-stranded DNA is integrated into the host chromosome to form a provirus. A small proportion of the viral DNAs form dead-end circular products, which nevertheless can serve as useful surrogate markers for monitoring viral replication. Utilizing real-time PCR technology, it is possible to track and quantify different stages of the reverse transcription process, the proviruses, and the nonintegrated dead-end reverse transcription products.