Establishment of carrier-state infection of a feline renal cell line with feline syncytial virus.
Establishment of carrier-state infection of a feline renal cell line with feline syncytial virus.
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DOI：
10.1292/jvms.57.65
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发表时间：
1995-02
期刊：
影响因子：
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通讯作者：
T. Miyazawa;S. Itagaki;K. Tomonaga;Y. Ikeda;T. Mori;Y. Kawaguchi;T. Gemma;Y. Tohya;M. Mochizuki-M.-Moch
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文献类型：
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作者：
T. Miyazawa;S. Itagaki;K. Tomonaga;Y. Ikeda;T. Mori;Y. Kawaguchi;T. Gemma;Y. Tohya;M. Mochizuki-M.-Moch
虽然猫合胞病毒（FSV）通常是高度致细胞病变的Crandell猫肾细胞，一个非致细胞病变的持续感染后，建立了最初感染的细胞与未感染的细胞共培养4次的细胞。90%以上的持续感染细胞呈FSV抗原阳性，电子显微镜显示培养物产生形态正常的FSV。载体培养物中的病毒具有感染性，但培养物中病毒的滴度低于细胞内感染的CRFK细胞。
Although feline syncytial virus (FSV) is normally highly cytopathogenic in Crandell feline kidney cells, a non-cytopathic persistent infection was established in the cells after cocultivation of the initially infected cells with uninfected cells 4 times. More than 90% of the persistently infected cells were positive for FSV antigen, and electron microscopy showed that the culture produced morphologically normal FSV. Virus from the carrier culture was infectious, however, the titer of the virus from the culture was lower than that from the cytocidally infected CRFK cells.