Development of Paper-strip Test for 3-Hydroxybutyrate and its Clinical Application
Development of Paper-strip Test for 3-Hydroxybutyrate and its Clinical Application
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3-羟基丁酸试纸条检测法的研制及其临床应用
DOI：
10.2337/diacare.7.5.481
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发表时间：
1984
期刊：
影响因子：
16.2
通讯作者：
Y. Shigeta
中科院分区：
文献类型：
--
作者：
Y. Harano;M. Suzuki;H. Kojima;A. Kashiwagi;H. Hidaka;Y. Shigeta
半定量 3-羟基丁酸酯 (3-OHBA) 的快速试纸测试方法已开发出来。将血清涂在纸条上后 5 分钟内显色，并通过目视或通过反射计读取紫色。它可以检测低至 0.1 mmol/L 至 2.0 mmol/L 的 3-OHBA 水平。浓度较高的样品可以通过连续稀释进行测量。临床实用性已在胰岛素依赖型糖尿病儿童夏令营以及常规糖尿病诊所进行了测试。夏令营早餐前，所有受试者的血清 3-OHBA 水平范围为 > 100 µumol/L 至 4 mmol/L。 4 名受试者中 3-OHBA 升高至 2-4 mmol/L 水平，硝普钠试验显示这 4 名受试者中只有 1 名出现酮尿。在糖尿病诊所，一项新的 3-OHBA 试纸测试显示 46 名糖尿病受试者中有 34 名 (74%) 患有酮血症，而硝普盐测试仅发现 4 名 (13%) 受试者患有酮血症。目前的 3-OHBA 纸条测试足够灵敏，可以检测低至 0.1 mmol/L 的水平，在临床上可用于快速检测酮症倾向以及监测糖尿病控制。
A rapid paper-strip test for the semiquantitating 3-hydroxybutyrate (3-OHBA) has been developed. The color develops within 5 min after applying the serum to the paper strip, and the purple color was read either visually or by reflectance meter. This can detect 3-OHBA levels as low as 0.1 mmol/L up to 2.0 mmol/L. The more concentrated sample can be measured on serial dilution. Clinical usefulness has been tested in a summer camp for insulin-dependent diabetic children as well as in a routine diabetes clinic. Serum 3-OHBA levels ranged from > 100 µumol/L to 4 mmol/L in all the subjects before breakfast in a summer camp. In four subjects, 3-OHBA was elevated to the level of 2–4 mmol/L, and only one of these four subjects exhibited ketonuria by nitroprusside test. In a diabetes clinic, a new paper-strip test for 3-OHBA has revealed ketonemia in 34 (74%) of 46 diabetic subjects, while nitro-prusside test revealed ketonemia in only 4 (13%). The present paper-strip test for 3-OHBA is sensitive enough to detect levels as low as 0.1 mmol/L and is clinically useful for rapid detection of ketosis proneness as well as for monitoring of diabetes control.