Replacing amino acids in translation: expanding chemical diversity with non-natural variants.
Replacing amino acids in translation: expanding chemical diversity with non-natural variants.
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在翻译中取代氨基酸：用非天然变体扩大化学多样性。
DOI：
10.1016/j.ymeth.2012.03.015
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Hartman,MatthewCT
中科院分区：
文献类型：
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作者：
White,ERailey;Reed,TimothyM;Ma,Zhong;Hartman,MatthewCT
在这里，我们描述了一种策略，用于合成肽与多个非天然氨基酸（UAA）使用体外翻译。我们的方法涉及去除天然氨基酸，并在重建的翻译系统中用UAA变体替换它。而其他系统需要工程组件或化学合成，以收费UAA到tRNA翻译前，我们的策略利用野生型机器和充电发生伴随翻译。该系统的设计允许容易地量化UAA的掺入效率和保真度。
Here, we describe a strategy for synthesis of peptides with multiple unnatural amino acids (UAAs) using in vitro translation. Our method involves removing a natural amino acid and replacing it with an UAA variant in a reconstituted translation system. Whereas other systems require engineered components or chemical synthesis to charge UAAs onto tRNAs prior to translation, our strategy utilizes the wild-type machinery and charging occurs concomitant with translation. The design of the system allows for easy quantification of the UAA’s incorporation efficiency and fidelity.