Human adult T-cell leukemia virus: molecular cloning of the provirus DNA and the unique terminal structure.
Human adult T-cell leukemia virus: molecular cloning of the provirus DNA and the unique terminal structure.
复制标题
人类成人 T 细胞白血病病毒：原病毒 DNA 的分子克隆和独特的末端结构。
DOI：
10.1073/pnas.79.22.6899
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发表时间：
1982
影响因子：
11.1
通讯作者：
M. Yoshida
中科院分区：
文献类型：
--
作者：
M. Seiki;S. Hattori;M. Yoshida
成人T细胞白血病病毒(ATLV)是一种与成人T细胞白血病密切相关的人类逆转录病毒。从病毒粒子RNA中克隆了整合的前病毒DNA和cDNA，并对其结构进行了分析。从成人T细胞白血病细胞与脐带淋巴细胞共培养建立的MT-1细胞系整合前病毒DNA克隆ATM-1，在病毒序列的两端分别含有约13,000个碱基对和长约8,000个碱基对的长末端重复序列。这两个LTR序列与细胞序列相连，其直接重复序列为7个核苷酸。每个LTR由754个碱基组成，末端有2个碱基的反向重复序列和T-A-T-A-A盒，其特征与其他已知逆转录病毒的LTRs相同。在5‘LTR附近有一个与小鼠白血病病毒tRNAPro结合位点相同的序列，提示tRNAPro是逆转录病毒基因组的一个引物。根据这些结构特征，推测ATLV的复制机制与其他已知的动物逆转录病毒相同。然而，RNA基因组末端的小末端重复序列的长度为228+/-1个碱基，远远长于迄今在禽类、小鼠或灵长类逆转录病毒中分析的长达80个碱基的长度。这些发现表明，ATLV应该与牛白血病病毒一起被归类为不同的逆转录病毒组，牛白血病病毒也会产生异常长的强STOP基因。
Adult T-cell leukemia virus (ATLV) is a human retrovirus closely associated with adult T-cell leukemia. The integrated provirus DNA and cDNA from virion RNA were molecularly cloned and their structures were analyzed. Clone lambda ATM-1 of an integrated provirus DNA in the MT-1 cell line, established from adult T-cell leukemia cells by cocultivation with cord lymphocytes, contained DNA about 13,000 base pairs (bp) long and long terminal repeats (LTR) at both ends of the viral sequence that were about 8,000 bp long. These two LTR sequences were linked to cellular sequences with direct repeats of 7 bp. Each LTR consisted of 754 bp including inverted repeats of 2 bp at the ends and the T-A-T-A-A box, characteristics in common with those of LTRs of other known retroviruses. Adjacent to the 5' LTR there was a sequence identical to the tRNAPro binding site in murine leukemia virus, suggesting that tRNAPro is a primer for reverse transcription of the viral genome. From these structural features, the mechanism of ATLV replication was suggested to be the same as that of other known animal retroviruses. However, the length of the small terminal repeats at the ends of the RNA genome, 228 +/- 1 bases, is much longer than the lengths, up to 80 bases, of those in avian, mouse, or primate retroviruses so far analyzed. These findings suggest that ATLV should be classified in a distinct group of retroviruses with bovine leukemia virus which also makes unusually long strong-stop cDNA.