Therapeutic potential of PPARα agonist in ligature-induced experimental periodontitis.
Therapeutic potential of PPARα agonist in ligature-induced experimental periodontitis.
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DOI：
10.1590/1678-7757-2021-0648
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hu Y
中科院分区：
文献类型：
--
作者：
Chen Y;Hu Y
炎症相关的免疫反应和骨代谢导致牙周炎患者牙齿大面积脱落。本研究旨在探讨过氧化物酶体增殖物激活受体(PPAR) α激动剂在体外和体内结扎诱导的实验性牙周炎中的抗炎作用。从C57BL/6J小鼠中分离脾细胞，在以下条件下培养48小时：对照，P. gingivalis脂多糖（LPS）（1µg/ml）；实验中，LPS(1µg/ml) + PPARα激动剂（非诺贝特）在1、10、50、100µM。RT-qPCR和ELISA分别检测TNF-α MRNA和分泌蛋白的表达水平。在C57BL/6J小鼠上颌第二磨牙周围扎丝结扎（7-0）2周。优化剂量的非诺贝特（50µM）和对照药于第3天、第6天和第9天注射到对侧腭龈。第14天，分别通过显微计算机断层扫描、抗酒石酸酸性磷酸酶（TRAP）染色和实时荧光定量PCR检测骨吸收、破骨细胞发生和牙龈TNF-α、IL-1β、IL-6和RANKL/OPG mRNA表达水平。与对照组相比，LPS显著增加了培养脾脏细胞中TNF-α的表达，非诺贝特在1-100µM剂量依赖性处理显著降低了TNF-α的表达（p<0.05）。注射非诺贝特后，与对照组相比，牙龈TNF-α、IL-1β、IL-6 mRNA水平及RANKL/OPG比值均显著降低（p<0.05）。与对照组相比，注射非诺贝特的一侧牙周骨丢失和TRAP阳性细胞形成明显减少（p<0.05）。一种抗炎治疗，PPARα激动剂，抑制结扎性实验性牙周炎的炎症和牙周骨丢失。
Inflammation-related immune responses and bone metabolism lead to extensive tooth loss in periodontitis. This study aims to investigate the effect of peroxisome proliferator-activated receptor (PPAR) alpha agonist anti-inflammatory treatment in vitro and in ligature-induced experimental periodontitis in vivo . Splenocytes were isolated from C57BL/6J mice and cultured for 48 hours under the following conditions: control, P. gingivalis lipopolysaccharide (LPS) (1 µg/ml); experimental, LPS (1 µg/ml) + PPARα agonist (fenofibrate) at 1, 10, 50, 100 µM. MRNA and secreted protein levels of TNF-α expression were detected by RT-qPCR and ELISA, respectively. Silk ligatures (7-0) were tied around maxillary second molars of C57BL/6J mice for two weeks. Optimized doses of fenofibrate (50 µM) and vehicle control were injected into the contralateral side of the palatal gingiva on days three, six, and nine. At day 14, bone resorption, osteoclastogenesis, and gingival mRNA expression levels of TNF-α, IL-1β, IL-6, and RANKL/OPG were measured by micro-computed tomography, Tartrate-resistant acid phosphatase (TRAP) staining, and Real-time quantitative PCR, respectively. TNF-α expression in cultured spleen cells were significantly increased in the presence of LPS, when compared with the control group, and significantly reduced by fenofibrate treatment in a dose-dependent manner from 1-100 µM (p<0.05). Gingival mRNA levels of TNF-α, IL-1β, IL-6, and the ratio of RANKL/OPG, were significantly decreased after injection of fenofibrate, when compared to the control side (p<0.05). Periodontal bone loss and TRAP positive cell formation were significantly decreased on the side with an injection of fenofibrate, as compared to the control side (p<0.05). An anti-inflammatory treatment, PPARα agonist, inhibited inflammation and periodontal bone loss in ligature-induced experimental periodontitis.
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过氧化物酶体增殖物激活的受体（PPAR）α激动剂非诺贝特保持骨骼量，而PPARγγ激动剂Pioglitazone在卵巢切除的大鼠中夸大了骨质流失。
影响因子：
2.7
作者：
Stunes AK;Westbroek I;Gustafsson BI;Fossmark R;Waarsing JH;Eriksen EF;Petzold C;Reseland JE;Syversen U
通讯作者：
Syversen U
影响因子：
3.5
作者：
Yu X;Hu Y;Freire M;Yu P;Kawai T;Han X
通讯作者：
Han X
DOI：
10.1177/10454411920030010501
发表时间：
1992-01-01
期刊：
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE
影响因子：
--
作者：
LAMSTER, IB;NOVAK, MJ
通讯作者：
NOVAK, MJ
影响因子：
7.6
作者：
Andriankaja, O. M.;Galicia, J.;Graves, D. T.
通讯作者：
Graves, D. T.
DOI：
10.17116/stomat201594511-16
发表时间：
2015-01-01
期刊：
Stomatologiia
影响因子：
--
作者：
Grigorovich, E Sh;Pomorgailo, E G;Stepanov, S S
通讯作者：
Stepanov, S S